粉防己碱联合谷胱甘肽对帕金森病大鼠的治疗作用

Therapeutical effect of Tetrandrine combined with Glutathione in rats with Parkinson's disease

目的探讨粉防己碱(Tet)联合还原型谷胱甘肽(GSH)对帕金森病(PD)大鼠的治疗作用。方法应用定向注射6-羟基多巴胺制作PD大鼠模型;将成功模型随机分为PD组、GSH组、左旋多巴(L-dopa)组、GSH+Tet+L-dopa组、GSH+Tet组,并给予相应药物腹腔注射治疗;给药后对各组大鼠进行阿朴吗啡旋转试验、脑组织黑质谷胱甘肽、纹状体单胺氧化酶B(MAO-B)含量测定;免疫组化法观察脑组织多巴胺能神经元数目;RT-PCR技术检测酪氨酸羟化酶(TH)mRNA含量。结果(1)L-dopa组、GSH+Tet+L-dopa组、GSH+Tet组阿朴吗啡试验旋转圈数较治疗前明显减少(P<0.05～0.01);(2)GSH+Tet组脑组织GSH含量明显高于其他治疗组,MAO-B含量明显低于其他治疗组(均P<0.05);(3)GSH+Tet组脑组织TH阳性神经元数目显著多于其他各治疗组(均P<0.05);(4)GSH+Tet+L-dopa组脑组织THmRNA含量高于其他治疗组及PD组(P<0.05～0.01)。结论Tet联合GSH能增强对PD大鼠的治疗作用。

Objective To investigate the therapeutical effect of Tetrandrine （Tet） combined with Glutathione （GSH） in rats with Parkinson＇s disease （PD）. Methods The PD model rats were established through stereotactic microinjection of 6- hydroxydopamine, then they were randomly divided into PD group, GSH group, L-dopa group, GSH ＋ Tet ＋ L-dopa group and GSH ＋ Tet group. The rats were peritoneal injected with corresponding medicines. The circling behavior of rats were observed induced by Apomorphine. The levels of GSH in substantia nigra and MAO-B in striatum were assayed after treatment, the numbers of dopaminergic neuron were observed by immunohistochemistry and the contents of TH mRNA were also be assayed in each group. Results （ 1 ） Compared with pretherapy, the rotation times of groups L-dopa , GSH ＋ Tet ＋ L-dopa , GSH ＋ Tet had statistical significance （ P 〈 0. 05 - 0. 01 ）. （2） GSH level of GSH ＋ Tet group was higher, MAO-B level was lower than those in other therapic groups （all P 〈0. 05 ）. （ 3 ） The number of tyrosine hydroxylase positive neurone in GSH ＋ Tet group was obviously more than that in other therapic groups （all P 〈 0. 05 ）. （4）TH mRNA levels in groups PD, GSH, L-dopa and GSH ＋ Tet ＋ L-dopa were all lower than that in GSH ＋ Tet group （ P 〈 0. 05 -0. 01 ）. Conclusion Tet combined with GSH can enhance the therapeutical effect in PD rats.