大鼠槲皮素灌胃后门脉血浆代谢组学研究

THE METABONOMIC STUDY ON PORTAL BLOOD PLASMA OF RATS AFTER ORAL ADMINISTRATION OF QUERCETIN

目的:研究槲皮素灌胃后对大鼠门脉血浆代谢组的影响。方法:健康雄性Wistar大鼠按40mg/kgbw剂量灌胃槲皮素,分别于灌胃前后0、0.5、1.0、2.0和4.0h采集门脉血液制备血浆,测定1H核磁共振谱,采用PLS-DA分析法对采集的数据进行分析。结果:灌胃槲皮素后,大鼠门脉血浆中二甲基亚砜水平明显升高,乳酸、葡萄糖含量呈现出先升高后逐渐恢复的趋势,谷氨酸、丙氨酸、琥珀酸、酮体水平也有所上升,柠檬酸、酪氨酸水平略有下降。结论:槲皮素灌胃后对肠道内源性代谢具有显著影响。

Objective： To investigate the effects of quercetin on metabolic profiles of portal blood plasma in rats. Method： Twenty five male Wistar rats were administered orally with 40 mg/kg bw quercetin. Portal blood was collected at 0, 0.5h, 1 h, 2 h and 4 h after dosing respectively and analyzed by ^1H NMR. The acquired data were subjected to partial least-squares discriminant analysis. Results： The identified biochemical effects associated with quercetin dosing included increased plasma concentrations of dimethyl sulfoxide, lactate, glucose, alanine, glutamate, succinate, β - hydroxybutyrate, acetone and decreased plasma concentrations of citrate and tyrosine. Conclusion： Quercetin may change the intestinal endogenous metabolism significantly in rats.