三种植物蛋白质酶解物对自发性高血压大鼠降血压效应的比较

THE COMPARATIVE STUDY ON ANTIHYPERTENSIVE EFFECTS OF THREE ISOLATED VEGETABLE PROTEIN HYDROLYSATES PREPARED WITH ALCALASE

目的:观察体外具有抑制血管紧张素转化酶(ACE)活性的绿豆、花生和大米分离蛋白的碱性蛋白酶alcalase酶解物的降血压效应。方法:通过模拟体内消化道环境研究经胃肠道消化酶处理对绿豆、花生和大米分离蛋白alcalase酶解物抑制ACE活性的影响,并利用自发性高血压大鼠(SHR)评定三种酶解物的体内降血压作用。结果:花生分离蛋白和大米分离蛋白alcalase酶解物经不同消化酶处理后活性均显著下降,绿豆分离蛋白酶解物经不同消化酶处理后其活性均有不同程度的提高。以600mg/kg bw的剂量灌胃后三种蛋白质酶解物对SHR均显示出降血压作用,绿豆最强,而花生最弱。三种蛋白质酶解物对SHR的心率均无影响。结论:绿豆、花生和大米分离蛋白质alcalase酶解物均具有降血压作用,但其体外抑制ACE活性与体内降血压效应不一致。

Objective： To investigate the antihypertensive effects of mung bean protein, peanut protein and rice protein alcalase hydrolysates with in vitro angiotensin I-converting enzyme （ACE） inhibitory activity in spontaneously hypertensive rats （SHR）. Method： The impact of digestive proteases on ACE inhibitory activity of hydrolysates of peanut, mung bean and rice protein isolates were evaluated under simulated gastrointestinal digestion and their antihypertensive effects were investigated in SHR after single oral administration. Results： All of three kinds of protein hydrolysates showed antihypertensive activities after single oral administration at a dose of 600 mg/kg bw, most potent in mung bean protein while least in peanut protein. There were no significant changes in the heart rate of SHR after oral administration of protein hydrolysates. Conclusion： Mung bean protein, peanut protein and rice protein hydrolysates all showed antihypertensive activity, but their potent inhibitory activities on ACE did not correlate with their antihypertensive activities found in SHR.