淫羊藿苷对Aβ_(25-35)所致神经细胞损伤的保护作用研究

Protection against Aβ_(25-35)-deprived injury in neurons by icariin

目的观察淫羊藿苷对β-淀粉样蛋白(Aβ25-35)所致神经细胞损伤的作用。方法18只Wistar大鼠简单随机分为5组,分别为假手术组、模型组、ICA药物组(30、60、120mg·kg-1),海马内注射Aβ25-3510μg(5μg.μl-1)制备AD大鼠模型,ICA30、60、120mg·kg-1连续灌胃给药14天后,石蜡切片HE染色观察海马神经元形态及数量的变化;神经细胞原代培养,MTT检测神经细胞增殖活性。结果与模型对照组比较,ICA连续灌胃给药14天可减少皮质和海马神经元的丢失;ICA给药组噻唑蓝值较模型组增加。结论淫羊藿苷对Aβ25-35所致神经细胞损伤具有保护作用,有利于老年性痴呆的治疗。

Objective To investigate the effect of icariin on Aβ25-35 - deprived injury in neurons. Methods The rats were randomized and divided into three groups of six animals each. They were sham group , model group and ICA groups （30,60,120mg.kg^-1）. Wistar rats were microinjected beta-amyloid peptide segment 25-35 （Aβ25-35） into hippocampus. Icariin （30,60,120mg.kg^-1,ig.） had been administered for fourteen days , Brain tissues were made into paraffin section of 6μm thickness and were stained with Hematoxylin-eosin to observe with optical microscope. The neurone were measured by MTT reduction test. Results The results of optical microscope and MTT reduction test showed that the number of hippocampal neurons in Icariin groups is more than that in model group. Conclusions Icariin could protect neurons against Aβ25-35 - deprived injury, the drug might be beneficial for the treatment of Alzheimer＇s disease.