草珊瑚组培中外植体消毒方法的研究

A protocol for explant sterilization of Sarcandra glabra in tissue culture

在建立草珊瑚组培快繁体系过程中，采用常规的表面消毒方法难以获得有效的无菌材料。本文报道了不同的表面消毒方法和抗生素结合使用对组培中污染菌的防除有较好作用。最有效的外植体消毒方案为：草珊瑚的幼嫩茎段经75％酒精处理30s后，用0．1％升汞溶液消毒10min，无菌水冲洗3～4次，然后浸于120mg·L^-1利福平溶液中振荡预培养2～4d，再用0．1％升汞溶液消毒10min，无菌水冲洗3～4次，可以达到较佳的消毒效果，消毒成功率可达55．56％。

During developing the rapid propagation system of Sarcandra glabra, it＇s very difficult to obtain plenty of sterilized materials by using conventional surface sterilization methods. A sterilization protocol for S. glabra rapid propagation is reported that a combination of surface sterilizations and antibiotic was effective to the elimination of contaminants in tissue culture. The most effective protocol： Firstly the explants were sterilized by immersion in 75 % ethanol for 30 s, and immersion in 0.1% HgCl2 for 10 min with agitation; then the explants were rinsed with sterile distilled water, and immersed in 120 mg· L^-1 rifampicin solution with gentle shaking for 2 - 4 d; Finally the explants were immersed in 0.1% HgCl2 again and rinsed with sterile water before transferred onto media. This protocol could obtain about 55.56% sterilized explants, which has been primarily employed in explant disinfection of micropropagation of Sarcandra glabra in vitro.