鲨鱼肝活性肽S-8300抗糖尿病与细胞保护作用

Study on the effect of anti-diabetes and cell protection of a new peptide S-8300

目的:探讨鲨鱼肝活性肽S-8300的抗糖尿病与细胞保护作用。方法:用流式细胞仪和原位缺口末端标记(TUNEL)法分析S-8300对链佐霉素(STZ)诱导小鼠糖尿病模型胰岛细胞凋亡的影响;用MTT法检测S-8300对损伤小鼠胰岛素瘤β-cell株(NIT-1)的影响。结果:S-8300能显著降低STZ诱导的胰岛细胞凋亡,对STZ损伤的小鼠胰岛素瘤β细胞具有明显的修复和保护作用。结论:S-8300的抗STZ损伤与其对凋亡细胞的保护作用有关,可能是其降血糖作用的机制之一。

AIM： The purpose of this study was to evaluate the effect of anti-diabetes and cell protection of a new peptide S-8300 on streptozotoein diabetic mice. METHODS： The apoptosis induced by streptozotoein （STZ） in the diabetic mice was tested through eytometer and TUNEL technology. The biological activity was measured by MTY assay with mouse insulinoma β-cell line （NIT-1） which was exposed to the toxins STZ. RESULTS： S-8300 obviously t^lueed the rate of apoptosis in injured pancreas induced by STZ and it could also protect the structure integrity and recover the damage of NIT-1 cell after exposed to the toxin of STZ CONCLUSION： The hypoglycemic mechanism of the anti-apoptotic effect of S-8300 may be related with the protective activity anainst the apoptosis.