BMP2真核表达载体的构建

Construction of BMP2 gene mammal expression plasmid

目的为研究骨缺损的基因治疗提供实验基础,构建人骨形态发生蛋白2真核表达载体。方法采用双酶切方法克隆载体将骨形态发生蛋白2基因与真核表达载体pcDNA3.1连接,构建pcDNA3.1-BMP2表达基因载体。结果通过测序分析证实,成功构建BMP2真核表达载体。结论成功构建BMP2表达载体。

Objective To clone and construct mammal expression vector for human bone morphogenetic protein 2 （BMP2）,in order to study gene .therapy for bone defect disease.Methods Plasmid pSPBMP2 was digested by Xba Ⅰ and Sal Ⅰ , the BMP2 gene was subcloned into pcDNA3.1. Results The mammal expression plasmid for BMP2, pcDNA3.1-BMP2, had been constructed. The cloned DNA BMP2 gene was confirmed by sequencing. Conclusion The mammal expression plasmid for BMP2 had been successfully constructed.