脐血浆对离体大鼠心肌缺血/再灌注损伤的保护作用

Protective effect of plasma from umbilical cord on myocardial ischemia/reperfusion injury in isolated rats

目的观察脐血浆(UC)对离体大鼠心肌缺血/再灌注损伤的保护作用并初步探讨其可能机制。方法取Wistar大鼠24只,随机分成正常对照组(Control)、缺血/再灌注损伤组(I/R)和脐血浆保护组(UC)。利用Langendorff离体灌流装置,复制大鼠心肌缺血/再灌注损伤模型。观察冠脉流出液中乳酸脱氢酶(LDH)活性、心肌组织匀浆中SOD活性及心肌超微结构等。结果与Control组相比,I/R组LDH活性明显增高,SOD活性降低,心肌超微结构损伤明显;脐血浆(1∶100)则明显减少LDH的漏出,同时使SOD活性升高及心肌超微结构损伤减轻。结论脐血浆能减轻大鼠心肌缺血/再灌注损伤,其机制可能与脐血浆具有抗氧化、清除自由基的作用有关。

Objective To observe the effect of human plasma from umbilical cord on myocardial isehemia/ reperfusion injury in rats and to study its possible mechanism. Methods Twenty-four Wistar rats were randomly divided into 3 groups： control group, isehemie reperfusion （I/R） group, and umbilical cord plasma （UC） group. Myocardial isehemia/reperfusion injury was induced using Langendorff perfusion system. The activity of LDH in coronary effluent and SOD in cardiac tissue, and the cardiac ultrastmcture were examined. Results Compared with the control group, the activity of LDH was increased, SOD enzyme activity was decreased, and injury of cardiac ultrastructure was obvious in I/R group; in UC （ 1 ： 100） group, the level of LDH was decreased and SOD enzyme activity was increased, the myocardial ultrastructure injury was improved, compared with I/R group. Conclusion The plasma of human could alleviate the myocardial ischemia/reperfusion injury in rats, and the underlying mechanism may be related to antioxidation and oxygen free radical scavenging of components in umbilical cord plasma.