iNOS在口腔鳞状细胞癌中的表达及其预后意义

EXPRESSION OF INOS AND ITS PROGNOSTIC SIGNIFICANCE IN ORAL SQUAMOUS CELL CARCINOMA

目的:调查诱导型一氧化氮合酶(iNOS)在口腔鳞状细胞癌(OSCC)中的表达及其预后意义。方法:48例OSCC经外科手术治疗后被分为两组:接受新辅助化疗24例及另外24例仅接受手术治疗。通过免疫组织化学方法评价了来自48例OSCC的经福尔马林固定、石蜡包埋预处理标本内的iNOS表达水平。结果:在48例OSCC中有47例被发现有iNOS染色,而在邻近正常口腔粘膜组织内却没有发现。在接受化疗者中,在高iNOS表达与颈部淋巴结转移之间存在一个显著性关系(P>0.05),而在仅接受手术治疗者中,低iNOS表达却与颈部淋巴结转移之间存在部分显著性关系。而且,高iNOS表达显著地与更高的生存率有关(P<0.01)。结论:iNOS过表达可能通过生成一氧化氮(NO)对肿瘤的发病起了重要作用。但是,在口腔癌中iNOS表达的临床意义仍然未明。为了全面建立起iNOS与肿瘤生物行为之间的关系,进一步的研究是必要的。

Objective：To investigate the expression of inducible nitric oxide synthase （iNOS） and its prognostic significance in oral squamous cell carcinoma （OSCC）. Methods：48 cases OSCC underwent surgical operation and were classified into 2 groups ： 24 cases with neoadjuvant chemotherapy and another 24 cases surgery alone. The level of iNOS expression was evaluated in formalin fixed paraffin embedded pretreatment specimens from 48 OSCCs by immunohistochemical method. Results：iNOS staining was detected in 47 of the 48 OSCCs but not in adjacent normal oral mucosal tissues. There was a significant correlation between high iNOS expression and cervical lymph node metas tasis in patients with chemotherapy （ P 〈 0.05）, whereas low iNOS expression subsignificantly correlated with cervical lymph node metastasis in cases surgery alone. Moreover, high iNOS expression significantly correlated with higher survival rate（P 〈0.01 ）. Conclusion： Overexpression of iNOS might make an important contribution to tumor formation through production of nitric oxide （NO）. However, the clinical importance of the expression of. iNOS in oral cancer remains unclear. Further research is required to fully establish the relationship between iNOS and biological tumor behavior.