MMP-11在胃癌患者血清中的表达及临床意义

Expression of MMP-11 in serum of gastric cancer and its significance

背景与目的:基质金属蛋白酶11(MMP-11)是基质金属蛋白酶家族中的一员,已有文献报道其与食管癌、肺癌等疾病的预后相关,尚缺乏MMP-11与胃癌的相关研究,本实验旨在观察基质金属蛋白酶11(MMP-11)在胃癌患者外周血清中的表达情况及临床意义。方法:收集2005年3月—2007年2月北京肿瘤医院收治的130例胃癌患者血清,采用逆转录-聚合酶链反应及酶联免疫技术检测MMP-11mRNA及蛋白表达情况。取20例正常健康人血清为对照组。结果:MMP-11mRNA在正常人群中表达的阳性率为5%,而在胃癌患者外周血清中阳性表达率(68.4%)高于正常人群;MMP-11蛋白在胃癌患者外周血清中表达量为(44±27)ng/ml,高于正常人群的(3±3)ng/ml;130例胃癌患者中,根治术后辅助治疗未见复发30例;术后复发或(和)转移的胃癌患者30例,复发、转移组血清MMP-11蛋白表达量(51±27)ng/ml,术后未见复发、转移的胃癌患者血清MMP-11蛋白表达量(27±28)ng/ml;两组患者外周血清MMP-11mRNA表达差异与蛋白水平一致。结论:MMP-11在胃癌患者外周血清中高表达,显著高于正常人群,尤其在根治术后转移和(或)复发的晚期患者的外周血中,推测其可能作为胃癌诊断、复发/转移的分子标志物。

Background and purpose： Matrix metalloproteinasel 1 （ MMP-11） is an interesting member of the MMP family, and its overexpression has been demonstrated in human cancers such as non-small cell lung cancer and oesophageal adenocarcinoma. Furthermore, strong MMP11 gene expression is correlated with both inereased aggressiveness of tumors and a poreclinieal outcome, but there was no related reports, on gastric cancer. We focussed our study cn the significance of MMP-11 expression in gastric cancer. Methods： MMP-11 was selected and confirmed at both mRNA and protein level in 130 cases of gaslrie cancer sennn specimen by RT-PCR, enzyme-linked-immuno-sorbent-assay （ELISA） lechniques, respectively. Results： The total positive rates of serum MMP-11 mRNA in gastric cancer and healthy people were 68.4vk and 5%. respectively. Our data demonstrated that MMP-11 protein levels in gastric cancer were （44 ± 27） ng/ml, significantly higher than those in control subjects （ P = 0. 001）. MMP-11 protein levels in 30 cases with recurrencc were （51 ± 27） ng/ml and （27 ± 28）ng/ml in 30 patienls without reculTcnee. MMP-11mRNA positive expression was consistent with M MP-11 protein lexels iH gastric cancer patiellts. Conclusions： Serum MMP-11 nlay play an important role as a biomarker in diagnosis and prediction of recun＇enl gastric cancer.