中国李组织培养过程中褐变的抑制研究

Study on browning control in the shoot-tip tissue culture of Chinese plum

研究了影响中国李（海湾红宝石）Prunus salicina Lindl．CV．Gulf ruby茎段培养褐变的因素，包括基本培养基组分、抗氧化剂、细胞分裂素等。结果表明，WPM作为基本培养基培养效果较好；维生素C1.0g·L^-1有效抑制褐变（褐变率16.7％），且能明显促进增殖（增殖倍数3.00）和生长；KT0.5-1.0mg·L^-1外植体生长良好，褐变减轻。适宜的培养方案为WPM＋IBA 0．1mg·L^-1 ＋ 6-BA／KT 0．5-1．0mg·L^-1 ＋ Vc 1．0g·L^-1，低温弱光下培养7d后转入光下继续培养。

Factors affecting browning of the stem segments culture of Chinese plum Prunus salicina Lindl. cv. Gulf ruby were studied with basal media, antioxidants and cytokinins. The results indicated that WPM as basal medium could achieve the better effect. 1.0g·L^-1 Vc availably depressed the browning of explants （ 16. 7% browning percentage ） and obviously enhanced the multiplication （3.00 multiplication time） and growth of explants. The growth of explants with 0.5 - 1.0mg·L^-1 KT was well,and a slight browning was observed in these explants. The feasible protocol to the stem segment culture of Chinese plum was that the half-lignification explants were cultured on WPM media including Vc 1.0g·L^-1 and IBA 0.1 mg·L^-1 added with either 6 - BA 0.5 - 1.0mg·L^-1 or KT 0.5 - 1.0mg·L^-1. Subsequently, the explants were placed under the conditions of low temperature and slight light. Seven days after the culture, the explants were transferred to light culture.