贵州省苗族、水族人群线粒体DNA RegionⅤ遗传多态分析

An Analysis on the Polymorphism in Region Ⅴ of Mitochondrial DNA in Miao and Shui People in Guizhou Province

目的:分析世居贵州省雷山县苗族、三都县水族人群线粒体DNA(mtDNA)RegionⅤ的遗传多态性。方法:采用聚合酶链反应-聚丙烯酰胺凝胶电泳(PCR-PAGE)法对91份苗族和96份水族样本的线粒体DNARegionⅤ区进行多态分析。结果:两个群体中均只发现标准型和短型(9bp缺失)两种多态,9bp缺失基因频率分别为:雷山苗族25.27%、三都水族25.00%。结论:上述两个少数民族群体中线粒体DNA RegionⅤ区短型多态基因频率较高,与其地域分布相一致。

Objective： To analyze the polymorphism in region Vof mitoehondrial DNA （mt DNA） in Miao people and Shui people in Guizhou. Methods： Polymerase chain reaction and polyacrylamide gel electrophoresis （PCR-PAGE） were used in the study. Results： Only two kinds of polymorphism were found in these populations. One belonged to standard pattern, and the other was short pattern（ namely 9 bp deletion）. The frequencies of short pattern in Miao and Shui people were 25.27% （23/91） and 25.00% （24/96） respectively. Conclusion： The frequencies of short pattern are relatively high in Miao and Shui people in Guizhou, which is in consistent with their regional distribution in other areas.