CT引导下热化疗对猪胰腺影响的实验研究

Experimental research of CT-guided thermochemotherapy for pig's healthy pancreas

目的探讨CT引导下热化疗治疗胰腺癌的可行性、安全性及疗效。方法正常家猪9头,CT引导下将18G穿刺针穿入小猪胰腺尾部。5-FU注射液750mg加热至80℃,维持时间不低于0.5h,2min内注入,按不同时间段分组,定期复查CT及MRI,并抽血化验血清淀粉酶,把不同时间段的大体病理与影像资料进行对比。结果经过CT引导下5-FU热化疗治疗后,正常小猪胰腺尾部均出现灶性坏死,第3、7和15d的大体病理与影像学表现吻合较好,实验小猪未出现肠粘连、梗阻等严重并发症。术后1h、3、7和15d复查血清淀粉酶,以第3天最高,其后为1h和第7天,到第15天降至正常。结论对于晚期胰腺癌患者,CT引导下热化疗可能为一种安全有效的治疗方法。

Objective To investigate the influence of CT-guided thermochemotherapy for pig＇s healthy pancreas and in turn to discuss the feasibility and efficacy on pancreatic tumors. Methods 9 healthy pigs were undergone CT-guided 80℃ themochemotherapy with 18 G fine meedle injecting individually of 5-Fu 750 mg（ 10ml）into the pancreatic tail within 2 min. CT, MRI and serum analyses test were carried out as the follow up study at 1 h, 3rd, 7th and 14th day after the procedure; since the 3rd day, 3 pigs were sacrificed right after every follow up and together with comparative study between diagnostic imagings and pathologic changes. Results The pig pancreatic tail necrotic lesions appeared to be most conspicuous at the 3rd day after thermochemotherapy and shrank gradually from 7th day to 14th day. Serum amylase level reached the highest at 3rd day after the treatment and lowered down at 7th day; and near approximately normal at 14th day. The outcomings of diagnostic imagings and macroscopic pathology were in accordance. Obvious pancreatitis occurred only in one pig and all others were nearly uneventful without serious complications of pancreatitis, intestinal adhesion and mortaligy. Conclusion CT-guided themochemotherapy can induce pancreatic, focal necrosis in healthy pig but with no serious complications; and these can further more establish the foundation for clinical practice.