植物光合能力检测中延迟荧光诱导参数优化

Optimization of Parameters to Obtain Maximum Delayed Fluorescence for Measuring Photosynthesis Capability of Plant

为优化延迟荧光诱导参数,以大豆(KeFeng No.1)叶片为样品,分析了影响延迟荧光强度的激发光强、激发时间和温度优化过程,并在优化条件下对样品进行了延迟荧光强度与相应净光合速率的对比测量。实验结果表明,优化条件下,两者具有极好的线性相关性(R1=0.989)。

The method based on delayed fluorescence（DF） to measure photosynthesis capadty has characteristics of accuracy and rapidity. The optimization of parameters to obtain maximum DF is an unavoidable problem that must be solved in order to promote the practicality of this method. In the paper, the leaves of soybean （Ke Feng No. 1） were taken as the model samples to illustrate this optimization process of parameters （excitation time,excitation intensity,and temperature） to obtain the maximal DF intensity in detail. The correlation of DF intensity and net photosynthesis rate（Pn） was achieved by the contrast experiments of DF intensity and Pn at the optimal conditions. The results showed that there was an excellent linear correlation between the DF intensity and Pn （R^2 =0.989）.