期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

hLRH-1调控结肠腺癌细胞SW480增殖的分子机制

Molecular Mechanism of hLRH-1 in Regulating Proliferation of Colon Adenocarcinoma Cells SW480
下载PDF
导出
摘要 目的初步探讨人核受体hLRH-1在结肠癌发生发展中可能的生物学功能。方法真核表达质粒pcDNA3-hLRH-1经脂质体介导转入SW480细胞中,RT-PCR和Western blot法分别检测外源基因mRNA及蛋白水平的表达;MTT法分析hLRH-1过表达对SW480细胞生长增殖的影响,同时行实时定量RT-PCR法分析细胞生长增殖相关基因Cyclin E1和Cyclin D1、以及凋亡调控相关基因Pten和Rb1的表达。结果hLRH-1过表达的SW480细胞增殖速度明显加快,同时其Cyclin E1基因的表达显著增高,而Pten和Rb1在hLRH-1过表达的SW480细胞中均呈明显的表达下调。结论外源hLRH-1的表达既通过上调Cyclin E1基因的表达而促进SW480细胞增殖,还可能通过调节Pten和Rb1基因的表达而参与细胞凋亡的调控。 Objective To explore the possible biological function of human nuclear receptor hLRH-1 in tumorigenesis and progress of colon cancer. Methods Plasmids pcDNA3-hLRH-1 were introduced into SW480 cells via lipofectamine. The expression of mRNA and protein of exogenous hLRH-1 was detected by RT-PCR and Western blotting, respectively. MTT assay was carried out to survey the proliferation of SW480 cells with overexpression of hLRH-1. Meanwhile, the expression of proliferation-related genes cyclin E1 and cyclin D1, and apoptosis-related genes PTEN and Rbl, were analyzed by real-time RT- PCR. Results The proliferation of SW480 cells were promoted under the condition of overexpression of hLRH-1. The expression of cyclin E1 was up-regulated significantly, while that of Pten and Rbl was down-regulated in SW480 cells with overexpressed hLRH-1. Conclusion The expression of exogenous hLRH-1 in SW480 cells induced the proliferation resulting form up-regulation of cyclin El, as well as participated in the regulation of apoptosis via influencing the expression of Pten and Rbl.
作者 郑英梦 王水良 兰风华
机构地区 南京军区福州总医院检验医学研究所分子医学研究中心
出处 《肿瘤防治研究》 CAS CSCD 北大核心 2007年第12期904-907,共4页 Cancer Research on Prevention and Treatment
基金 福建省青年科技人才创新资助项目(2004J067) 福建省自然科学基金计划资助项目(C0710038) 福州总医院院立课题资助项目(200638)
关键词 核受体hLRH-1 结肠癌 外源基因表达 增殖 Nuclear receptor hLRH-1 Colon cancer Expression of exogenous gene Proliferation
分类号 R735.35 [医药卫生—肿瘤]
  • 相关文献

参考文献12

  • 1Fayard E, Auwerx J, Schoonjans K. LRH-1: an orphan nuclear receptor involved in development, metabolism and steroidogenesis [J]. Trends Cell Biol, 2004, 14(5): 250-260.
  • 2Gu P, Goodwin B, Chung AC, et al. Orphan nuclear receptor LRH-1 is required to maintain Oct4 expression at the epiblast stage of embryonic development [J]. Mol Cell Biol, 2005, 25 (9) : 3492-3505.
  • 3Nitta M, Ku S, Brown C, et al. CPF.- an orphan nuclear receptor that regulates liver-specific expression of the human cholesterol 7alpha-hydroxylase gene [J]. Proc Natl Acad Sci USA, 1999, 96(12).- 6660-6665.
  • 4del Castillo-Olivares A, Gil G. Alpha 1-fetoprotein transcriplion factor is required for the expression of sterol 12alpha hydroxylase, the specific enzyme for cholic acid synthesis. Potential role in the bile acid-mediated regulation of gene transcription [J].J Biol Chem, 2000, 275(23): 17793-17799.
  • 5Luo Y, Liang CP, Tall AR. The orphan nuclear receptor LRH-1 potentiates the sterol-mediated induction of the human CETP Gene by Liver X Receptor [J]. J Biol Chem, 2001, 276 (27) : 24767-24773.
  • 6Krylova IN, Sablin EP, Moore J, et al. Structural analyses reveal phosphatidyl inositols as ligands for the NR5 orphan receptors SF-1 and LRH-1[J]. Cell, 2005, 120(3): 343-355.
  • 7Schoonians K, Dubuquoy L, Mebis J, et al. Liver receptor homolog 1 contributes to intestinal tumor formation through effects on cell cycle and inflammation [J]. Proc Natl Acad Sci USA, 2005, 102(6): 2058-2062.
  • 8王水良,傅继梁.核受体的研究进展[J].Acta Genetica Sinica,2004,31(4):420-429. 被引量：26
  • 9Botrugno OA, Fayard E, Annicotte J, et al. Synergy between LRH-1 and β-Catenin induces G1 cyclin-mediated cell proliferation [J]. Mol Cell, 2004, 15(4) : 499-509.
  • 10Annicotte JS, Chavey C, Servant N, et al. The nuclear receptor liver receptor homolog-1 is an estrogen receptor target gene [J]. Oncogene, 2005, 24(55): 8167-8175.

二级参考文献69

  • 1Beato M, Herrlich P, Schutz G. Steroid hormone receptors: many actors in search of a plot. Cell, 1995,83: 851-857.
  • 2McKenna N J,O' Malley B W. Combinatorial control of gene expression by nuclear receptors and coregulators. Cell, 2002,108:465-474.
  • 3Clarke N D, Berg J M. Zinc fingers in Caenorhabditis elegans:finding families and probing pathways. Science, 1998, 282:2018-2022.
  • 4Ashburner M,Chihara C,Meltzer P,Richards G. Temporal control of puffing activity in polytene chromosomes. Cold Spring Harbor Symp Quant Biol, 1974,38:655-662.
  • 5Wong C W,McNally C,Nickbarg E,Komm B S,Cheskis B J. Estrogen receptor-interacting protein that modulates its nongenomic activity-erosstalk with Src/Erk phosphorylation cascade. Proc Natl Acad Sci USA ,2002,99:14783～14788.
  • 6Kousteni S,Bellido T,Plotkin L I,OBrien C A,Bodenner D L,Hah L,Han K,DiGregorio G B, Katzenellenbogen J A, Katzenellenbogen B S,Roberson P K,Weinstein R S, Jilka R L, Manolagas S C. Nongenotropic, sex-nonspecific signaling through the estrogen or androgen
  • 7Dean M,Hamon Y, Chimini G. The human ATP-binding cassette (ABC) transporter superfamily. J Lipid Res, 2001,42: 1007-1017.
  • 8Willson T M, Brown P J, Sternbach D D, Henke B R. The PPARs..from orphan receptors to drug discovery. J Med Chem,2000,43:527-550.
  • 9Schrader W T,Kuhn R W,O' Malley B W. Progesterone-binding components of chick oviduct: Receptor B subunit protein purified to apparent homogeneity from laying hen oviducts. J Biol Chem,1977,252:299-307.
  • 10Wrange O, Okret S, Radojcic M, Carlstedt-Duke J, Gustafsson J A. Characterization of the purified activated glucocorticoid receptor from rat liver cytosol. J Biol Chem, 1984,259:4534-4541.

共引文献25

肿瘤防治研究
2007年 第12期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部