摘要
目的研究肝细胞肝癌(hepatocellular carcinoma,HCC)血窦样腔隙中中整合素β1亚单位(inte-grin-β1,CD29)、血管细胞粘附分子(vascular cell adhesion molecule-1,VCAM-1,CD106)的表达意义。方法在90例HCC中分别采用免疫组化(SP)法检测VCAM-1和免疫组化双标双染(DouSP)法检测in-tegrin-β1,PCNA的表达。采用多元协方差分析指标之间的相关性。结果HCC中integrinβ1和VCAM-1主要表达在血窦样腔隙中,增殖癌细胞核表达PCNA。integrinβ1在癌旁组织的表达高于HCC。HCC中VCAM-1表达的阳性率为76.67%,与病理分级正相关。多元协方差分析显示肝癌不同分级组integrin-β1均与VCAM-1的表达正相关,且低分级组integrin-β1和VCAM-1均与癌增殖PCNA表达显著相关并随分级增高其相关性减小。双标双染显示integrin-β1与PCNA的双表达在HCC中显著高于癌旁。结论integrin-β1和VCAM-1在HCC发生、发展、增殖与分化中是一个较强的影响因素。癌血窦样腔隙中VCAM-1蛋白表达有助于对HCC癌增殖和病理分级的判断。
Objective To study the significance of the expressing of imegrin-β1（CD29）, vascular cell adhesion molecule-1（VCAM-1,CTD106） in sinusoid- lemons of the hepatocellular carcinoma（HCC） .Methods In 90 cases of HOE, the expression of VCAM-1 protein was detected by immunohistochemistry techniques SP, and that of integrin-β1 and PCNA proteins were detected by DouSP. The relativity of three facors were analysed with Multifactoral covariance metthod.Results In HOE, integrin-β1 and VCAM-1 were expressed in sinusoid- lemons primarily, proliferating cell nuclear expressed PCNA. The expression of integrin-β1 was stronger in the pericarcinoma than in HOE. The positive rate of VCAM-1 expressed in HOE was as high as 76. 67%, and was positively related to patholngical grading. Multifactoral covariance analysis results shows the expression of integrin-β1 were all positively related to that of VCAM-1 in different grading groups, and they were significantly associated with PCNA of HOE in low-grading group, but the latter＇s relativity descreased in high-grading group. DouSP results： the m-expression of CD29 and PCNA was significantly much higher in HOE than in pericarcinoma tissues, Conclusion Integrin-β1, VCAM-1 are stronger factors on the occurrence,development, proliferation and differerented of HCC. The expression of VCAM-1 is helpful to evaluating diagnosis and proliferation of HCC.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2007年第12期958-961,987,共5页
Cancer Research on Prevention and Treatment
