气相色谱-原子吸收联用技术测定人体体液中烷基汞

Determination of Alkylmercuric Compounds in Human Body Fluids by Gas Chromatography-Atomic Absorption Spectrophotometry

建立了一种用于生物样品中分子形态分析的气相色谱与原子吸收联用系统。系统的转移线为聚四氟乙烯管，组分在色谱住内分离后，经转移线运送到原子吸收检测系统。实验证明:系统具有灵敏度高、选择性好的优点，例汞绝对检测限为10-2ng，并首次用于人体血液和尿液中烷基隶的两种形态即氯化甲基汞和二甲基汞的定性和定量分析。

In order to detect trace of alkylmercuric compounds in human body fluids, a highly sensitive, selective and rapid method, based on the coupling of gas chromatography with atomic absorption spectrophotometry, has been developed. The transfer-tube was made up of Teflon, with stainless steel tubes in the two sides. The atomization of alkylmercuric compounds was performed in quartz tube.The quantitative determination of alkylmercuric compounds in urine and blood samples collected from the workers exposing to metallic mercury, has been extensively carried out in this work. The optimal Westoo program was used to enrich methylmercuric chloride in samples, Extracts of methylmercuric chloride in benzene were determined by GC-AAS, with a total recovery of more than 90%. Dimethylmercury in samples was extracted once by xylene. Then the extracts were determined, with a total recovery of 65%-70%. The detectable limit for mercury was O. 01ng.The methylation of mercury was confirmed in our experiment. This method can be used for the determination of other metal1ic compounds.