一种新型L-脯氨酸衍生的高效液相色谱手性固定相

A NOVEL CHIRAL STATIONARY PHASE DERIVED FROM L PROLINE FOR LIQUID CHROMATOGRAPHIC RESOLUTION OF CHIRAL COMPOUNDS

设计和制备了一种用于手性化合物的高效液相色谱拆分的新型手性固定相（ＣＳＰ）。该固定相以构象刚性的Ｌ－脯氨酸为手性选择子，经引入保护基、萘酰胺化、加氢脱保护基、酰胺化和与ＬｉＣｈｒｏｓｏｒｂＮＨ２５μｍ微粒硅胶偶联等５步反应而制得。在此ＣＳＰ结构中引入萘酰胺，可增强ＣＳＰ和手性溶质之间的π－π相互作用，以提高该ＣＳＰ的对映体选择性。报道了４种Ｎ－３，５－二硝基苯甲酰－ＤＬ－氨基酸甲酯对映体色谱拆分的初步结果，对映体选择性在１．０４～１．１８之间。色谱拆分条件的优化和该固定相的应用将有待于进一步研究。

The design and preparation of a novel chiral stationary phase (CSP) were described. The chiral resolutions of N 3,5 dinitrobenzoyl DL amino acid methyl esters were performed on the CSP, The selectivities were between 1 04 and 1 18. The applications of the CSP and the optimization of the chromatographic conditions will be further studied.