大豆细胞质雄性不育恢复基因的SSR标记

IDENTIFICATION OF SSR MARKERS LINKED TO THE FERTILITY RESTORER GENE FOR THE CMS IN SOYBEAN

大豆细胞质雄性不育系的获得,为大豆杂种优势利用奠定了基础。在确认RN型大豆细胞质雄性不育系属单基因配子体不育,恢复性是由显性单基因控制的遗传模式的基础上,开展恢复基因的分子标记研究,旨在找到与恢复基因紧密连锁的分子标记,为进一步克隆恢复基因及恢复系的分子标记辅助育种奠定基础。研究选用412对SSR引物,利用RN型不育系YA与恢复系167杂交的F2分离群体,获得了与恢复基因连锁的两个标记Satt414和Satt596,遗传距离分别为16.4和14.6cM。为了找到更近的分子标记,分析了Satt414和Satt596附近的所有SSR引物,并利用两个遗传差异较大的亲本重新构建了分离群体,从而获得了与恢复基因连锁比较紧密的标记Satt547,遗传距离为7.56cM。根据Cregan等构建的大豆分子遗传连锁图,将恢复基因定位于J连锁群上。

The soybean hetero was controlled were ge F2 development of cytoplasmic male sterile（CMS）line has sis. It was confirmed that the fertility restoration of the by a single dominant nuclear gene. Two SSR makers, netically 1 facilitated the utilization of RN type soybean CMS 1 Satt414 and Satt596,wh ne ch nked to the nuclear restorer gene,were selected by screening of SSR markers segregation population from the cross between sterile line YA and restorer line 167. The genetic distance between the markers and the restorer gene were 16.4 and 14.6 cM,respectively. A segregating population developed from a cross between more diversified parents was used for screening the closer SSR markers. SSR marker Satt547 with genetic distance of 7.56 cM to the restorer gene was identified. The nuclear restorer gene was located in J linkage group according to Cregan＇s soybean genetic map.