摘要
根据转基因大豆的内源基因Lectin和外源基因CaMV35S的序列设计特异引物和探针,利用这些引物和探针,采用先定性后定量的方法对6份大豆加工产品转基因成分进行了定量检测。结果显示有两份样品含有转基因成分,其转基因成分含量分别为1.4%和0.4%。
According to the DNA sequences of endogenous Lecin gene and exogenous CaMV35S gene,primers and probes was designed. Using the primers and probes, transgenic components of six unknown samples were qualitatively detected followed by quantitatively detected. The results showed only two samples of them were positive and the content of transgenic components was 1.4 % and 0.4 %, respectively.
出处
《大豆科学》
CAS
CSCD
北大核心
2007年第6期939-942,共4页
Soybean Science
关键词
转基因大豆
定量检测
PCR
Transgenic soybean
Quantitative Detection
PCR
