高效液相色谱法测定复方感冒液多组分含量

Determination of Multicomponent Contents in Anticold Solution by High Performance Liquid Chromatography

报道了采用高效液相色谱法（ＨＰＬＣ）测定复方感冒液中愈创木酚、扑热息痛、甲基麻黄碱、咖啡因、美沙芬及扑尔敏的含量。色谱柱为ＺｏｒｂａｘＯＤＳ柱，前４种药物采用的流动相为甲醇－０．０５ｍｏｌ／Ｌ磷酸二氢钾－磷酸（２５０∶７５０∶０．５），检测波长２１４ｎｍ；后２种药物采用的流动相为甲醇－水－磷酸－十二烷基硫酸钠（８０∶２０∶０．５∶０．２），检测波长２６０ｎｍ。在此色谱条件下，６种药物可获得很好的分离，回收率也高。

A high performance liquid chromatographic method has been successfully used to separate and determine the six drugs in an anticold solution. In the anticold solution, potassium guaiacol sulfonate, acetaminophen, D,L methylephedrine, and caffeine were analyzed with a column of Zorbax ODS, by using the mobile phase of MeOH 0.05mol/L KH 2PO 4 H 3PO 4(250∶750∶0.5) and UV detector at 214nm, dextromethorphan HBr and chlorpheniramine maleate were analyzed with the same column, by using the mobile phase of MeOH H 2O H 3PO 4 SLS(80∶20∶0.5∶0.2) and UV detector at 260 nm. This method is proved to be very effective to separate the six drugs mentioned above. For potassium guaiacol sulfonate, acetaminophen, D,L methylephedrine, caffeine, dextromethorphan HBr and chlorpheniramine, the response values were linear between 1 0 10μg, 5 0 50μg, 0 25 2 5μg, 0 5 5μg, 0 25 2 5μg, 0 05 0 5μg respectively and the recoveries were 100 53%±1 12%, 100 19%±0 58%, 99 4%±1 60%, 99 55%±1 01%, 99 37%±1 52%, 99 01%±1 20% (n=5) respectively.