用高效液相色谱法测定热应激时大鼠肺及肝组织中细胞膜磷脂的变化

Determination of Changes in Membrane Phospholipids of Rat Lung and Liver Tissues from Acute Heat Stress by High Performance Liquid Chromatography

应用高效液相色谱法（ＨＰＬＣ）测定了正常大鼠肺及肝组织中细胞膜磷脂的含量及热应激时膜磷脂的含量变化。流动相为甲醇∶乙腈∶８５％磷酸（３∶１００∶１，Ｖ／Ｖ／Ｖ），色谱柱为μ－Ｐｏｒａｓｉｌ柱（３．９ｍｍｉ．ｄ．×３００ｍｍ）。通过测定膜磷脂的变化。

The changes in the contents of phosphatidylinositol(PI), phosphatidylserine(PS), phosphatidylethanolamine(PE) and phosphatidylcholine(PC) in lung and liver membrane tissues of rats suffered from acute heat stress were investigated with HPLC. The separation was performed on a μ Porasil Si column, 300mm×3.9mm i.d., by isocratic elution of acetonitrile∶methanol∶85% phosphoric acid (100∶3∶1). The phospholipids were detected at 205nm. The results showed that the contents(mean±SD) of PI, PS, PE and PC in normal rats liver were 270.0±18.2μg/g,133 7± 8 6μg/g, 1465±132μg/g and 53.7± 2.8mg/g respectively, whereas those in heat stressed rats whose rectal temperature was increased to 42℃ and maintained for 15 minutes were decreased by 14% 25%(P<0 01) except PS. When the heat stressed rats recovered to room temperature for 4h, the contents of phospholipids returned to normal level. The changes of PS and PC in rat lung tissues were identical with those in liver tissues.