摘要
本文利用薄层层析－干柱层析技术对石油卟啉化合物的分离制备进行了研究，最佳分离条件为，以１０～４０μｍ薄层层析硅胶Ｇ为固定相，以石油醚∶甲苯∶二氯甲烷＝３∶１∶１为展开剂，展开方式为上行和下行，卟啉的Ｒｆ值为０．５２～０．６３，可制备ｍｇ级卟啉样品供给后续的高效液相色谱和质谱进行分子水平的分析。
The separation and preparation of petroleum porphyins using TLC LC technique were studied.The optimum operating conditions were:10～40μm silica gel(G) as the stationary phase and petroleum ether/toluene/dichloromethane(3∶1∶1) as the eluent.The results showed that the R f values of porphyrins were 0.52 to 0.63.Samples of porphyrins of the order of magnitude of mg could be prepared with this method for the purpose of further analysis on a molecular level by HPLC and MS.
出处
《分析试验室》
CAS
CSCD
1997年第2期39-41,共3页
Chinese Journal of Analysis Laboratory
基金
自然科学基金
关键词
石油
卟啉
薄层色谱
柱色谱
层析法
Biology marker
petroleum porphyrins
thin layer chromatography (TLC)
column chromatography(LC)
