七种嘧啶碱和嘌呤碱的胶束电动毛细管色谱分离研究

Studies on the Separation of 7 Pyrimidine and Purine Derivatives by Micellar Electrokinetic Capillary Chromatography

研究了影响七种核酸碱基－胸腺嘧啶，尿嘧啶，胞嘧啶，腺嘌呤，鸟嘌呤，黄嘌呤和次黄嘌呤毛细管电泳分离的因素。在２４．５℃下，七种碱基在４０ｍｍｏｌ／Ｌ十二烷基硫酸钠－１０ｍｍｏｌ／Ｌ硼砂－１７％甲醇－３．０ｍｍｏｌ／Ｌβ－环糊精（ｐＨ９．３０）缓冲液中电泳可得良好分离。保留时间的变异系数小于１．８％（ｎ＝６）

The retention behaviours of seven common bases thymine,uracil,cytosine,adenine,guanine,xanthine and hypoxanthine were investigated by micellar electrokinetic capillary chromatography.At 24.5℃ and 20kV applied voltage,7 bases introduced into the separation carillary (65cm×50 μm i.d.,51.5cm to UV detector)were separated satisfactorily within 21 minutes in 40 mmol/L SDS 10mol/L borax 17% methanol 3 0mmol/L β cyclodextrin buffer solution (pH9.30).The detection limits of the bases were within 0.6～6.0mg/L,and the relative standand deviation of retention times and UV absorbances of the bases were less than 1.8% and 6.5% respectively.