偏侧PD猴模型黑质HMW MAP-2表达的变化

Changes of HMW MAP-2 expressing in substantia nigra of hemiparkinsonism monkey model

目的探讨偏侧帕金森病(Parkinson's disease,PD)猴模型黑质高分子量微管相关蛋白-2(HMW MAP-2)表达的变化。方法对4只恒河猴经单侧颈内动脉注射1-甲基4-苯基1,2,3,6-四氢吡啶(MPTP)制备成偏侧PD猴模型后,应用免疫组化染色方法,观察4只偏侧PD猴模型和2只正常猴黑质HMW MAP-2表达的变化。结果偏侧PD模型猴MPTP毁损侧的黑质的HMW MAP-2免疫活性较毁损侧对侧和正常对照猴明显降低,但MPTP毁损侧对侧的黑质的HMW MAP-2免疫活性未发现明显变化。结论偏侧PD猴模型黑质的HMW MAP-2表达减少可能对黑质神经元的变性和死亡起重要作用。

Objective To investigate the expressing changes of high molecular weight microtubule associated protein 2 （HMW MAP-2） in substantia nigra of the hemiparkinsonism monkey model. Methods Four hemiparkinsonian monkey models were induced by injecting 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine （MPTP） unilaterally into the internal carotid artery. The expressing changes of HMW MAP- 2 of substantia nigra in four hemiparkinsonism monkey model and two normal control monkeys were observed by immunohistochemistry. Results The immunoreactivity of HMW MAP- 2 were decreased significantly in the MPTP lesioned side substantia nigra of hemiparkinsonian monkey models compared with those in contralateral to the MPTP lesioned side and normal control monkey. But, no significant changes were found in substantia nigra contralateral to the MPTP lesioned side. Conclusions The expressing decrease of HMW MAP-2 in the substantia nigra of hemiparkinsonian monkey models, which may play the important role in the mechanism of neuronal degeneration and death.