谷氨酰胺预处理对大鼠肠缺血再灌注后肺损伤的影响

Effects of preoperatively glutamine administration on rat lung injury induced by intestinal ischemia/reperfusion

目的探讨谷氨酰胺预处理对大鼠肠缺血再灌注后肺损伤的影响。方法建立大鼠肠缺血再灌注损伤模型,观察谷氨酰胺预处理对大鼠肺组织细胞间黏附分子(ICAM-1)、热休克蛋白-70(HSP-70)基因表达、髓过氧化物酶(MPO)、丙二醛(MDA)水平以及肺组织病理变化的影响。结果谷氨酰胺预处理可以增强大鼠肺组织HSP-70的表达,抑制ICAM-1的表达,降低肺组织MPO、MDA水平。结论谷氨酰胺预处理对大鼠肠缺血再灌注后肺组织具有保护作用,其机制可能与诱导HSP-70基因表达有关。

Objective To evaluate effects of pretreatment with glutamine on lung injury induced by intestinal ischemia/reperfusion （II-R） in rats. Methods Glutamine or saline were injected through tail vein before the model of intestinal ischemia/reperfusion in rats were established. The gene expression of intercellular adhesion molecule-1 （ICAM-1） and heat shock protein-70 （HSP-70） were tested with RT-PCR methods. The levels of heat shock protein-70 in the lung were measured with Western Blotting. Myeloperoxidase and malondi-aldehyde and pathological changes were also measured. Results The gene expression of heat shock protein-70 was enhanced by pretreatment with glutamine, and the level of HSP-70 was parallelly increased. Nevertheless, MPO, MDA and the gene expression of ICAM-1 were inhibited. Conclusion Pretreatment with glutamine can lessen the lung injury induced by intestinal ischemia/reperfusion in rats, the induction of HSP-70 gene may be one of the potential mechanisms.