NDRG1在乳腺浸润性导管癌中的表达及其与侵袭、转移的关系

Expression of N-myc downstream regulated gene 1 in breast invasive duct carcinoma and its relationship with tumor metastasis and invasive potential

目的研究NDRG1(N-myc downstream regulated gene 1)基因在乳腺浸润性导管癌中的表达,及其与乳腺癌侵袭、转移的关系。方法应用免疫组化法检测26例伴淋巴结转移的乳腺浸润性导管癌和45例无淋巴结转移的乳腺癌中的NDRG1、MMP2、MMP9、TIMP2、E-cad的表达状态。结果乳腺癌伴淋巴结转移的标本中NDRG1的表达率低于无淋巴结转移标本(P<0.01)。NDRG1蛋白的表达与TIMP2的表达正相关(P<0.05);而与MMP2、MMP9和E-cad的表达无关(P>0.05)。结论NDRG1与浸润性导管癌的侵袭和转移能力密切相关,可能是一个候选的肿瘤转移抑制基因,并且可能成为乳腺癌的一个有用的预后指标。

Objective To study the correlation of expression of N-myc downstream regulated gene 1 （ NDRG1 ） with invasion of breast invasive duct carcinoma and lymph node metastasis. Methods A total of 71 specimens including 26 case of primary breast invasive duct carcinoma with lymphnode metastasis, 45 case of non-metastasis breast invasive duct carcinoma were observed. NDRG1 was detected by immunohistochemistry in formalin-fixed and paraffin-embedded sections. At the same time, the correlations of NDRG1 with E-cad, MMP2, MMP9, and TIMP2 were investigated. Results The expression of NDRG1 in breast cancer with metastasis of lymph nodes （9/26） was lower than that of non-metastasis of lymph nodes （32/45） （ P 〈 0.01 ）. NDRG1 was positively correlated with TIMP2 （ P 〈 0.05）, whereas there was no significant relationship between NDRG1 and E-cad, MMP2, MMP9 in breast cancer. Conclusion NDRG1 protein may play an important role in determine the metastasis and invasion ability of breast invasive duct carcinoma. NDRG1 may be a candidate metastasis suppressor gene for breast cancer and may serve as a useful prognostic marker of breast carcinoma.