排毒增益汤对弓形虫感染雄鼠非特异免疫和体液免疫功能的变化的影响

The Effect of Paiduzengyi Soup on the Non-specific Immunity and Humoral Immunity of Male Mice Infected by Toxoplasma gondii

目的:观察排毒增益汤对弓形虫感染雄鼠非特异免疫和体液免疫功能的影响,并探讨其作用机制。方法:采用AH株弓形虫包囊感染8W龄雄鼠造模,设立正常对照组、模型组、乙胺嘧啶阳性药物组、排毒增益汤大、中、小剂量组。分别用MTT法和间接ELISA法测定脾NK细胞杀伤率、B淋巴细胞转化率及IgG抗体和亚类含量。结果:排毒增益汤各剂量组脾NK细胞杀伤率、B淋巴细胞转化率及IgG抗体和亚类含量均高于模型组(P<0.05)。结论:排毒增益汤具有提高弓形虫感染雄鼠非特异免疫和体液免疫功能的作用。

Objective： To observe the effect of Paiduzengyi soup on the immune function of male mice infected by toxoplasma gondii, and to study the mechanism. Methods： Male model mice of 8-week were induced by AH toxoplasma gondii. All the model mice were divided into control group, model group, pyrimetharpme group, high, medium and low doses of Paiduzengyi soup group. The method of MTT and indirect ELISA was used separately to test the killing activity of NK cells, the proliferation rate of B lymphocytes, IgG antibodies, IgG2 and IgG1. Results： The killing rate of NK cells, the proliferation rate of B lymphocytes, and the content of IgG antibodies, IgG2 and IgG1 of Paiduzengyi soup groups of different doses were higher than those of the model group. Conclusion： Paiduzengyi soup can obviously improve non-specific immunity and humoral immunity of male mice infected by toxoplasma gondii.