角膜上皮细胞的免疫学特性

The immunological character of corneal epithelial cells

目的:探讨角膜上皮细胞刺激淋巴细胞增生的能力和共同刺激通路分子在角膜上皮细胞的表达。方法:体外共同培养人角膜上皮细胞和外周血单个核细胞,免疫组织化学染色方法测定HLAⅡ类抗原、CD40、CD154、CD80和CD86的表达,同时对比角膜移植排斥反应的移植片。荧光辅助细胞筛选、分析、测定CD69的上调。结果:HLAⅡ类抗原在角膜上皮细胞表达,γ-干扰素能够升高共同刺激通路分子CD40和CD80的表达;而且HLAⅡ类抗原和CD40均在角膜移植排斥反应的移植片上被检测出。T淋巴细胞在共同培养系统中被上皮细胞激活。结论:人角膜上皮细胞参与角膜移植排斥反应过程,移植免疫排斥反应被上皮细胞所诱导和初始化,但最终反应作用于内皮细胞。

Objective：To investigate the capabihty of corneal cells to stimulate lymphocyte proliferation and the expression of costimulatory molecules on primary human corneal epithelial cells. Methods：Human corneal epithelial cells were cocultivated with peripheral blood mononuclear cells（PBMC）. Expression of HLA class Ⅱ antigens, CD40, CD154, CD80 and CD86 was measured by immunohistochemieal staining, and the expression of such molecules above on rejected corneal grafts was contrastingly assessed. Activation of lymphoeytes was determined by measuring upregulation of CD69 by FACS analysis. Results： HLA II expression was detectable on human primary corneal epithelial cells, and furthermore, upregualtion of costimulatory molecules CD40 and CD80 were also found after induction with γ-interferon. Both of HLA and CD40 were expressed on rejected corneal grafts. T lymphocytes were activated by epithelial cells in the coculture system. Conclusion： Human corneal epithelial cells are involved in rejection process of the corneal transplantation. The immunological reaction is triggered by epithelial cells ,and endothelial cells suffer as the targets.