生物衍生骨支架材料的制备及其体内组织相容性的研究

Preparation of bio-derived bone and its histocompatibility

目的:制备生物衍生骨支架材料,研究其生物安全性和生物相容性,从而为骨组织工程提供最佳的支架材料。方法:制备生物衍生骨支架材料,将BALB/c小鼠分为三组,分别为对照组、生物衍生骨支架植入组和异种骨植入组。植入21天后分别采用肌肉刺激实验,刀豆蛋白A(ConA)诱导的脾淋巴细胞转化实验和补体依赖性细胞毒实验分析生物衍生骨对动物机体局部组织的影响和免疫功能的影响。取植入物周围组织做HE染色进行组织学分析。结果:支架组材料周围未见明显的炎症反应,而异种骨组骨组织周围有大量的炎细胞浸润,并有坏死组织。支架组的脾淋巴细胞转化实验和补体依赖性细胞毒实验结果与对照组相比较均无明显差异;而异种骨组结果则均明显高于对照组,具有显著性差异。结论:生物衍生骨支架材料无细胞毒性,具有良好的组织相容性。

Objective：To prepare the bio-derived bone and then to study its biological security and compatibility. Methods： The BALB/c mice were divided into three groups： control group, bio-derived bone group and xenogenic bone group, which were respectively implanted into muscles of mice. After 21 days, the local histological and immunological effect were investigated by muscle stimulation test, lymphocyte blastogenesis test and cytotoxicity test. Results：There was no obvious inflammatory reaction in muscles around the bio-derived bone. But there were a large numbers of inflammatory cells and necrotic tissues around the xenogenic bone. The results of cytotoxicity test were consistent with the lymphocyte blastoagenesis test. The results of the bio-derived bone group were not significantly different from the control group（ P 〉 0. 05）. However the results of the xenogenic bone group were higher than those of the control group（P 〈 0.05）. Conclusion ：The bio-derived bone had good histocompatibility without cytotoxicity.