骨髓单个核细胞与多孔乳酸制备的混合物修复家兔关节软骨缺损

Bone marrow monocytes and porous lactic acid mixture in the repair of rabbit articular cartilage defects

目的:骨髓间充质干细胞用于软骨缺损修复方面的研究已有较多报道,实验以聚乳酸为载体,拟进一步验证骨髓单个核细胞与多孔乳酸混合物对关节软骨缺损的修复效果。方法:实验于2006-11/2007-06在华北煤炭医学院实验中心完成。①实验材料及分组:清洁级3至4个月龄家兔30只,雌雄不限,体质量2.0～3.0 kg。②实验过程及分组:参考文献制备多孔乳酸载体,抽取5只家兔骨髓,分离液分层,取单个核细胞体外培养.制备细胞-多孔乳酸混合物。取家兔25只随机分为3组,即细胞-多孔乳酸组17侧膝关节,缺损中植入细胞-多孔乳酸混合物;空白多孔乳酸组17侧膝关节,缺损中植入空白多孔乳酸混合物;未处理组16侧膝关节,缺损不作任何处理。③实验评估:分别于术后4,8,12周麻醉状态处死动物,进行大体及组织学切片观察。结果:实验过程家兔死亡4只,进入结果分析21只42膝。①大体观察:术后12周,细胞-多孔乳酸混合物组标本缺损处修复组织的色洋呈乳白色,但与周围正常软骨的界限已变得模糊不清;空白多孔乳酸混合物组与未处理组缺损处充填物的色泽与周围软骨组织类似,表面平坦,但边界仍清晰可辨。②组织学评分:术后4,8,12周,细胞-多孔乳酸混合物组的修复效果均优于其他两组(P<0.05)。结论:利用体外培养的骨髓单个核细胞-多孔乳酸混合物修复兔关节软骨缺损,缺损均由类透明组织修复,表明其对关节软骨具有修复能力。

AIM： There are many studies on mesenchymal stem cells for cartilage defect. The study verified the repair outcome of bone marrow monocyte and porous lactic acid mixture on articular cartilage taking polyactic acid as a carrier. METHODS： Experiments were performed at the Experimental Center of North China Coal Medical University from November 2006 to June 2007. ①Thirty clean rabbits aged 3-4 months, weighting 2.0-3.0 kg, of either sex, were selected. ②Porous lactic acid carriers were made according to the references. Bone marrow was collected from five rabbits, separated with separating medium to harvest monocytes for in vitro culture, and then to prepare cell-porous lactic acid mixture. Twenty-five rabbits were randomly divided into three groups. Cell-porous lactic acid mixture was implanted into the defects in the ceU-porous lactic acid group （17 knees）. Blank porous lactic acid mixture was implanted into the defects in the blank porous lactic acid group （17 knees）. No intervention was performed in the non-treatment group （16 knees）. ③Rabbits were executed under drugged state 4, 8 and 12 weeks after the surgery. General and histological observations were performed. RESULTS： Four rabbits died during the experiment, and 21 rabbits （42 knees） were involved in the result analysis. ①12 weeks after the surgery, defected tissues were ivory in the cell-porous lactic acid group, and similar to the normal cartilage. The edge of the defective tissues and the normal cartilage was clear in the blank porous lactic acid group and non-treatment group. ②4, 8 and 12 weeks after the surgery, the effect of cell-porous lactic acid group on defective tissue repair was better than the other two groups （P 〈 0.05）. CONCLUSION： Bone marrow monocyte cells in combination with porous lactic acid mixture can repair articular cartilage and the defect is repaired with transparent-like tissues.