改良消蚀法制备脱细胞真皮基质微粒及其生物相容性评价

Preparation of ADM granule by improved solution method and its biocompatibility tests

目的:利用改良消蚀法制备一种新型脱细胞真皮基质(Acellular dermal matrix,ADM)微粒,并对其相容性加以评价,为组织工程微粒皮肤的制备奠定研究基础。方法:应用机械法切除猪真皮乳头层,结合消蚀法和冻融法除去网状真皮中的所有细胞制备成ADM,将ADM搅碎成颗粒状,对其做细胞毒性实验和皮下埋藏实验检验其相容性。结果:该方法制备的ADM微粒韧性好,细胞去除完全,胶原三维稍松散但结构完整,无基底膜和乳头层。细胞毒性实验显示其基本无毒性,成纤维细胞在ADM微粒上增殖良好,皮下埋藏试验未见明显排异反应,ADM微粒能诱导血管和成纤维细胞长入。结论:用改良消蚀法制备的ADM微粒抗原性低、相容性好,可以作为组织工程微粒皮肤的支架和填充材料。

Objective To prepare a new kind of xenogeneic acellular dermal matrix （ADM） granules with improved solution method and test its biocompatibility for establishment of tissue engineering granule skin. Methods The xenegeneic ADM was made from the reticular layer of porcine dermis and prepared by solution and freeze thawing methods. Its biocompatibility was tested by cytotoxicity test,cell culture and subdermal implantion. Results Intact cells were absent from the ADM by the improved method and the tenacity was good;The collagn fibers were a little loose in arrangement but their structure were normal;There were no basement membrane and dermal papillary layer retained , and amounts of xenogeneic were lower. The ADM granule was nontoxic, fibroblasts grew and proliferated well on it and there were no immune rejection in the subdermal implantation. The ADM granules could induce the infiltration of host cells and the vascularization. Conclusion The ADM granules prepared by improved solution method possessed very low antigenicity and good biocompatibility and could be a good scaffold for tissue engineering granule skin for further study.