摘要
Male Chinese loaches were exposed to 17β-estradiol (E2) and nonylphenol (NP) both singly and in combination for 42 days using semi-static waterborne exposure system. Plasma vitellogenin (Vtg) was chosen as determining endpoint. The results demonstrated that 0.5 μg/L E2 could induce the enhancement of Vtg contents in male Chinese loaches after exposure for 21 days, which showed a time-related increasing manner; NP was also estrogenic to male Chinese loach, and the vitellogenic responses showed in a time-and dose-related manner, which was less potent than that of E2. The bi- nary mixtures of E2 and NP can significantly elicit the production of Vtg in male Chinese loaches, which was more potent than that of individual compounds, and Vtg induced in the binary mixture groups was higher than the summation of Vtg induced in the corresponding single-compound groups at the same concentration.
Male Chinese loaches were exposed to 17β-estradiol （E2） and nonylphenol （NP） both singly and in combination for 42 days using semi-static waterborne exposure system. Plasma vitellogenin （Vtg） was chosen as determining endpoint. The results demonstrated that 0.5 μg/L E2 could induce the enhancement of Vtg contents in male Chinese loaches after exposure for 21 days, which showed a time-related increasing manner; NP was also estrogenic to male Chinese loach, and the vitellogenic responses showed in a time- and dose-related manner, which was less potent than that of E2. The binary mixtures of E2 and NP can significantly elicit the production of Vtg in male Chinese loaches, which was more potent than that of individual compounds, and Vtg induced in the binary mixture groups was higher than the summation of Vtg induced in the corresponding single-compound groups at the same concentration.
基金
Supported by the State High Tech Development Plan (Grant No. 2006AA06Z424)
National Basic Research Program of China (Grant No. 2003CB415001)
National Natural Science Foundation of China (Grant No. 40503014)
Chinese Academy of Sciences (Grant No. KZCX3-SW-431)
关键词
化学相互作用
内分泌
17Β-雌二醇
卵黄蛋白原
chemical interaction, Chinese loach, endocrine disruption, 17β-estradiol, Misgumus anguillicaudatus, nonylphenol, vitellogenin
