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PD98059对肝癌HepG2细胞Tec信号转导作用的初步研究(英文) 被引量：1

Effect of MEK1 inhibitor PD98059 on the signal transduction of Tec in HepG2 hepatoma cells
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摘要 Objective: To investigate the effect of MEK1 inhibitor PD98058 on Tec and ERK2 in HepG2 hepatoma cells. Methods: The expression of mRNA and protein of Tec and ERK2 in HepG2 cells was detected by immunocytochemistry as- say. After various concentration of PD98059 treatment, the expression of Tec and ERK2 mRNA in HepG2 cells was detected by RT-PCR and Western blotting. Results: Tec and ERK2 expressed highly in HepG2 cells. PD98059 obviously inhibited the expression of mRNA and protein of Tec and ERK2 in a dose-dependent manner, in which 40 μmol/L of PD98059 exhibited the strongest inhibiting effect. Conclusion: PD98058, as MEK1 inhibitor, can inhibit Tec, block the signal route of Ras/Raf/ERK and to impede the signal transduction in HepG2 cells. Tec may be the signal protein in the upper stream of Ras/Raf/ERK in hepatocarcinoma cells and is supposed to interact with the signal way of Ras/Raf/ERK. Objective： To investigate the effect of MEK1 inhibitor PD98058 on Tec and ERK2 in HepG2 hepatoma cells. Methods： The expression of mRNA and protein of Tec and ERK2 in HepG2 cells was detected by immunocytochemistry assay. After various concentration of PD98059 treatment, the expression of Tec and ERK2 mRNA in HepG2 cells was detected by RT-PCR and Western blotting. Results： Tec and ERK2 expressed highly in HepG2 cells. PD98059 obviously inhibited the expression of mRNA and protein of Tec and ERK2 in a dose-dependent manner, in which 40 μmol/L of PD98059 exhibited the strongest inhibiting effect. Conclusion： PD98058, as MEK1 inhibitor, can inhibit Tec, block the signal route of Ras/Raf/ERK and to impede the signal transduction in HepG2 cells. Tec may be the signal protein in the upper stream of Ras/Raf/ERK in hepatocarcinoma cells and is supposed to interact with the signal way of Ras/Raf/ERK.
作者 Jijun Zheng Chuan Chen （Co-First Author） Ge Wang Jing Deng Jin Yang Hongzhong Wang Minzhi Zhang Qiong Li Wen Xu Qing Hu Zengpeng Li Dong Wang
机构地区 Cancer Center Department of Cell Biology
出处 《The Chinese-German Journal of Clinical Oncology》 CAS 2007年第6期542-545,共4页 中德临床肿瘤学杂志（英文版）
基金 Supported by grants from the National Natural Science Foundation of China (No. 30370341, 30570410) the Academic Foundation for Au-thors of National Excellent Doctoral Dissertation of China (No. 200261).
关键词 肿瘤 肝癌 治疗方法 转换模式 PD98059 Tec ERK2 hepatocarcinoma signal transduction
分类号 R735.7 [医药卫生—肿瘤]
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参考文献14

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同被引文献14

  • 1Fatty Liver and Alcoholic Liver Disease Study Group of the Chinese Liver Disease Association..非酒精性脂肪性肝病诊疗指南[J].中华肝脏病杂志,2006,14(3):161-163. 被引量：1510
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  • 6Alessi D R, Cuenda A M, Cohen P, et al. PD98059 is a specific inhibitor of the activation of mitogen-actived protein kinase in vitro and in vivo[J]. J Biol Chem,1995,270(46) :27489 -94.
  • 7Yang S Q, Lin H Z, Mandal A K, et al. Disrupted signaling and inhibited regeneration in obese mice with fatty hvers: implications for nonalcoholic fatty liver disease pathophysiology [ J ]. Hepatology,2002,35(3 ) :731 -2.
  • 8Carmen G Y, Victor S M. Signalling mechanisms regulating lipolysis[J]. Cell Signal,2006,18(4) :401 -8.
  • 9Tsai J, Qiu w, Kohen-Avramoglu R, et al. MEK-ERK inhibition corrects the defect in VLDL assembly in HepG2 cells: Potential role of ERK in VLDL-apoB100 particle assembly[ J ]. Arterioscler Thromb Vasc Biol,2007,27(1) :211 -8.
  • 10Watanabe M, Inukai K, Katagifi H, et al. Regulation of PPAR gamma transcriptional activity in 3T3-L1 adipocytes [ J ]. Biochem Biophys Res Commun,2003,300 (2) :429 - 36.

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The Chinese-German Journal of Clinical Oncology
2007年 第6期

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