摘要
Objective: To study the expression of connexin43 (Cx43) gene and its correlation with the apoptosis related genes Bcl-2 and Bax in transitional cell carcinoma of the bladder (BTCC), and to investigate the role of Cx43 in the BTCC. Methods: Reverse transcription polymerase chain reaction was used to detect the expression of Cx43 mRNA and immunocytochemistry technique was used to detect the expressions of Bcl-2 and Bax proteins in 60 cases of BTCC tissues, and compared with that of 15 cases of pericancerous tissues and 15 cases of normal bladder tissues. Results: The positive expression rate of Cx43 mRNA in 60 cases of BTCC tissues was 43.33% which was significantly lower than that in pericancerous tissues (73.33%) and normal tissues (100%) (χ2 = 17.58, P < 0.01). The expression of Cx43 had significant negative correlation with the pathological degree and lymph node metastasis of BTCC (χ2 = 9.33 and 9.74, respectively, P < 0.01). However, no correlation was found between the expression and patient sex, age, clinical staging and diameter and growth pattern of BTCC (P > 0.05). Expression of Cx43 negatively correlated with Bcl-2 protein (r = -0.63, P < 0.01) and positively correlated with Bax protein (r = 0.52, P < 0.01). Conclusion: The down-regulated expression of Cx43 gene was closely associated with the development, invasion and metastasis of BTCC. It could be used as a prognostic indicator for BTCC. Cx43 gene may have antagonistic effects with Bcl-2 gene and synergic with Bax gene in the initiation and progression of BTCC.
Objective： To study the expression of connexin43 （Cx43） gene and its correlation with the apoptosis related genes Bcl-2 and Bax in transitional cell carcinoma of the bladder （BTCC）, and to investigate the role of Cx43 in the BTCC. Methods： Reverse transcription polymerase chain reaction was used to detect the expression of Cx43 mRNA and immunocytochemistry technique was used to detect the expressions of Bcl-2 and Bax proteins in 60 cases of BTCC tissues, and compared with that of 15 cases of pericancerous tissues and 15 cases of normal bladder tissues. Results： The positive expression rate of Cx43 mRNA in 60 cases of BTCC tissues was 43.33% which was significantly lower than that in pericancerous tissues （73.33%） and normal tissues （100%） （χ^2 = 17.58, P 〈 0.01）. The expression of Cx43 had significant negative correlation with the pathological degree and lymph node metastasis of BTCC （χ^2 = 9.33 and 9.74, respectively, P 〈 0.01）. However, no correlation was found between the expression and patient sex, age, clinical staging and diameter and growth pattern of BTCC （P 〉 0.05）. Expression of Cx43 negatively correlated with Bcl-2 protein （r = -0.63, P 〈 0.01） and positively correlated with Bax protein （r = 0.52, P 〈 0.01 ）. Conclusion： The down-regulated expression of Cx43 gene was closely associated with the development, invasion and metastasis of BTCC. It could be used as a prognostic indicator for BTCC. Cx43 gene may have antagonistic effects with Bcl-2 gene and synergic with Bax gene in the initiation and progression of BTCC.
关键词
蛋白质
细胞
膀胱癌
治疗方法
bladder neoplasms
transition cell carcinoma
connexin
prognosis
