衰老进程中大鼠淋巴细胞凋亡率的比较研究及淫羊藿总黄酮的干预作用

Comparative studies of the apoptosis rates of lymphocytes in the progress of aging and interventional effect of EF on aged rats

目的:比较研究衰老进程中各年龄段大鼠淋巴细胞凋亡率的差异及淫羊藿总黄酮(EF)对老年期细胞凋亡的干预作用。方法:选用SD大鼠脾脏分离的淋巴细胞为研究对象,用流式细胞方法,比较从出生后3天直到27月龄衰老进程中,各代表性年龄段的细胞凋亡率差异,并观察EF对老年过度细胞凋亡的影响作用。结果:刚出生3天组的凋亡率最低,随着增龄,细胞凋亡率逐渐升高,仅10月龄组例外,其凋亡率小于4月龄组,27月龄的细胞凋亡率最高。EF干预后,27月龄组的细胞凋亡率明显下降,介于10月龄与18月龄之间水平,以上差异均有显著统计学意义(P<0.05～P<0.001)。结论:在衰老进程中,大鼠淋巴细胞的凋亡率随增龄而逐渐升高,在老年大鼠存在过度凋亡现象,而EF可抑制老年淋巴细胞过度凋亡。

Objective： Compare the apoptosis rates of lymphocytes in the progress of aging rats and interventional effect of EF. Methods：The lymphocytes derived from SD rat spleen were employed as the studyed cells and were observed by flow cytometry. The differences of apoptosis rates in groups from 3d to 27m were emphasized in the study. The aim of this study of effect of EF on excessive apoptosis of aging rats was to found foundation for the following study. Results：3-day newborn rat group had the lowest apoptotic percentage. The extent of apoptosis correlated with prolongation of age but an exception in 10m group, which had a lower apoptotic percentage than 4m group. The highest apoptotic percentage existed in 27m. When intervened with EF, the apoptosis ratio of 27m group apparently decreased to the level between 10m and 18m. All of the data were statistically significant（ P 〈 0. 05 or P 〈 0. 001 ）. Conclusion：The apoptosis rate of rat lymphocytes gradually has been increased and correlated with the time of aging. NF-κB plays a key role in aging. EF could inhibit the excessive apoptosis of lymphocytes in aged rats.