摘要
利用A-PAGE(Acid-polyacrylamide gel electrophoresis)技术研究了43份来自四川、西藏的青稞(裸大麦)育成品种醇溶蛋白的遗传多样性。结果表明:43份材料共分离出22种相对迁移率不同的醇溶蛋白谱带,41种不同的电泳图谱,说明供试材料醇溶蛋白的遗传多态性丰富。就遗传多样性差异来说,在醇溶蛋白位点I,四川>西藏(P<0.01);在位点Ⅱ,四川<西藏(P<0.01);在位点Ⅲ,四川<西藏(P>0.05)。四川材料的遗传多样性平均值略高于西藏材料,差异不显著。四川、西藏材料间的遗传分化较低。聚类分析结果表明,43份材料可分成A、B、C3大类,材料聚类与其生长的地区有明显的相关性。四川材料间、西藏材料间的平均遗传距离较低。在超级青稞育种中,应发掘和利用新的优异青稞资源,扩大青稞的遗传基础。
Genetic diversitys of hordein of 43 accessions developed hulless barley varieties, included 25 entries from Sichuan and 18 entries from Tibet of China, were analyzed by acid -polyacrylamide gel electrophoresis (A- PAGE). The results showed that, a total of 22 hordein bands with relatiely different migration rate and 41 types of hordein patterns were identified in these materials, which indicated that richer genetic polymorphism of hordein existed in the developed hulless barley varieties. With regard to genetic diversity difference, Sichuan 〉 Tibet ( P 〈 0. 01) at the hordein locus Ⅰ, Sichuan 〈 Tibet (P〈0. 01) at Ⅱ and Sichuan 〈Tibet (P〉0. 05) at Ⅲ. The mean of genetic diversity in the accessions from Sichuan was slightly higher than that from Tibet without obvious difference. While, lower genetic differentiation between Sichuan and Tibet accessions was also detected. All the accessions were divided into three major groups: A, B and C by cluster analysis using UPGMA, which indicated that the obvious correlation between the clusters of the accessions and their growing regions was presented. The mean of genetic distance both between the accessions from Sichuan and Tibet was also low. Thus, it was suggested that the excavation and use of new excellently hulless barley resources should be strengthened to widen genetic basis of hulless barley resoures in over - hulless barley breeding.
出处
《大麦与谷类科学》
2007年第4期1-6,共6页
Barley and Cereal Sciences
基金
四川省科技厅应用基础项目
四川农业大学青年科技创新基金项目资助
关键词
大麦
青稞
醇溶蛋白
遗传多样性
Barley
Hulless barley
Hordein
Genetic diversity