摘要
研究了博莱霉素A5(BLMA5)与DNA作用前后的光谱性质及初步的动力学性质,发现BLMA5可催化DNA氧化断链,而且符合米氏动力学.通过对BLMA5的催化效率、底物专一性以及温度、pH、金属离子对BLMA5催化DNA断链反应影响的研究,发现BLMA5的作用行为在许多方面与酶的催化行为非常类似,提出BLMA5是一种小分子生物催化剂的看法.
The 5aG strain virus glycoprotein gene was inserted into the vaccinia virus transfection vector pJ120,which the coding sequence was controlled under the vaccinia virus late promoter P11.Plasmid DNA was introduced into vaccinia virus Tiantan strain by LIPOFECTIN method.Through the homologous recombination,this gene was integrated into TK region of vaccinia virus genome. Under BrdU selection pressure,one strain of recombinant virus has been picked out.Immunofluorecence test and Western Blotting showed that the lecombinant virus expressed 5aG strain rabies virus glycoprotein on the surface of infected cell.The molecular weight of glycoprotein approximated 64×10 3.Mice experiments showed that all the mices which immunized with recombinant virus acquired protection against lethaldose of CVS strain rabies virus challenge intracerebally.After mice was inocubated with recombinant virus, the titer of neutralizing antibody againt rabies virus was as high as 1:560 on the 14th day.
出处
《武汉大学学报(自然科学版)》
CSCD
1997年第4期517-522,共6页
Journal of Wuhan University(Natural Science Edition)
基金
国家教委留学回国人员科研基金
湖北省自然科学基金
关键词
博莱霉素A5
类酶性
生物催化剂
抗肿瘤抗生素
aG rabies virus glycoprotein gene,Vaccinia virus of Tiantan strain, gene expression