高效液相色谱法测定白花丹参中总丹参酮和丹参酮ⅡA含量

Determination of Total Tanshinone and TanshinoneⅡA in Salvia Miltiorrhiza Bge.f. Alba by HPLC

目的采用高效液相色谱（HPLC）法对白花丹参总丹参酮和丹参酮ⅡA含量进行分析。方法采用乙醇超声提取法提取白花丹参中脂溶性成分,HPLC法进行测定,色谱柱为Waters symmetry shieLd RP18（105 mm×3.9 mm,5μm）;测定总丹参酮以甲醇-水（90：10）为流动相,测定丹参酮ⅡA以乙腈-水（75：25）为流动相,流速均为0.8 mL·min^-1;检测波长270 nm。结果总丹参酮在0.075～0.750μg·mL^-1浓度内与峰面积线性关系良好,线性方程为A=5.88×106C-1.99×105,r=0.999 4（n=5）,丹参酮ⅡA在7.5～50.00μg·mL^-1内线性关系良好,线性方程为A=7.8×104C-5.41×104,r=0.999 4（n=5）。结论建立的HPLC法灵敏度高,方法准确,测定结果重现性好。白花丹参中含有丰富的总丹参酮和丹参酮ⅡA。

Objective To determine total tanshinone and tanshinone Ⅱ A in Salvia miltiorrhiza bge. f. alba by HPLC. Methods Ultrasonic extraction with 90% ethanol was used to extract the lipid soluble component in Salvia miltiorrhiza bge.f. alba. The liquid chromatography was carried out by using waters symmetry shield RP18 column （ 150 mm ×3.9 mm,5 μm） with detection wavelength at 270 nm. The mobile phase was 90% methanol in determining the total tanshinone,75% acetonitrile in determining the tanshinone H A. The flow rate was 0.8 mL · min^-1. Results There was a good linear relationship within the range of 0.075 - 0. 750 mg · mL^-1 （ total tanshinone） and 7.5 - 50.0 μg · mL^-1 （ tanshinone H A） （ r = 0. 999 g, n = 5 ）. Conclusion The method is simple, rapid, selective and sensitive. Total tanshinone and tanshinone Ⅱ A are rich in Salvia miltiorrhiza bge. f. alba.