表面等离子体共振成像生物芯片检测系统

Construction Basis of Biotic Gene Chip Checking System with Surface Plasmon Resonance Imaging

根据表面等离子体共振(Surface Plasmon Resonance,SPR)原理,提出基于表面等离子体共振成像(Surface Plasmon Resonance imaging,SPRI)的生物芯片检测系统构建方法.介绍了SPRI生物芯片检测系统的原理、自行组建的SPRI生物芯片检测系统的结构.采用Kretschmann型棱镜耦合结构激励SPR,偏振的平行光经棱镜投射到生物芯片上,发生表面等离子体共振,由CCD摄像机采集反射光芯片图像.以巯基修饰淋病奈瑟氏菌探针为例验证该系统,利用自组装单分子层技术(Self-Assembled Monolayer,SAM)固定探针.应用该检测系统采集了探针共振、非探针处共振、探针和非探针处都不共振时的生物芯片图像.

Utilizing the principle of Surface Plasmon Resonance, a construction method for biochips detecting systems based on Surface Plasmon Resonance imaging is presented. The principle of SPRI biochips detecting systems and the structure of systems are provided. A Kretschmann prism configuration is used to stimulate SPR. Polarized parallel light is projected to the sensor through the prism. The reflected biochip image is collected by CCD camera. As an experiment, Mercapto to modify Neisseria gonorrhoeae probe is used to prove the system. A Self-Assembled Monolayer （SAM） is affixed to the probe in which 6-Mercaptohexanol is blocked. The system collected all the biochip images of probe and non-probe resonances and non-resonances.