血管抑素基因转染抑制胆囊癌细胞的实验研究

Empirical study of angiostatin transfected gene therapy for gallbladder cancer

目的:观察血管抑素基因转染对体外人胆囊癌细胞系GBC-SD细胞株增殖及血管抑素表达和裸鼠致瘤体积的影响,初步探讨血管抑素基因转染抑制胆囊癌细胞生长的可能性。方法:应用pcDNA3.1(+)-angiostatin基因转染GBC-SD胆囊癌细胞。观察细胞生长情况,制作细胞生长曲线;Western-blot法分析血管抑素的表达情况;裸鼠种植瘤模型观察肿瘤的体积和质量。结果:pcDNA3.1(+)-angiostatin基因转染的GBC-SD胆囊癌细胞生长明显受到抑制,血管抑素的蛋白表达也明显增加。转染的肿瘤细胞在裸鼠种植瘤明显小于阴性组和空白组。结论:血管抑素基因转染可能具有抑制胆囊癌细胞增殖及生长的作用。

Objective ：To observe angiostatin transcription affects generation,angiostatin expression of human gallbladder cancer cell line GBC - SD in vitro and its effect on volume of transplanted tumor and explore the potential value of angiostatin gene therapy for gallbladder cancer. Methods ： The recombinant vector pcDNA3.1 （ ＋ ） - angiostatin was transfected into human gallbladder cancer cell line GBC - SD with Lipofectamine 2000, and paralleled with the vector and mock control. Cell growth curves were plotted ; Angiostatin protein expression was determined by Western -blot. The transfected and untransfected cells were implanted subcutaneously into nude mice. The volume and quality of tumors was musured. Results： Growth of human gallbladder cancer cell line GBC -SD transfected with pcDNA3.1 （ ＋ ） - angiostatin was obviously inhibited. Angiostatin protein expression was increased. In nude mice model, volume and quality of tumors were markedly inhibited in experimental group as compared to controls. Conclusion： Angiostatin may have ability of suppressing human gallbladder cancer cell proliferation and growth in vitro and in vivo.