Ang-2、CD105和CD34在脑胶质瘤中的表达及生物学意义

Expessions of Ang-2,CD105 and CD34 in gliomas and their biological significance

目的:探讨促血管生成素-2(Ang-2)、CD105和CD34在脑胶质瘤中的表达及生物学意义。方法:应用免疫组化SP法检测45例胶质瘤和10例正常脑组织中Ang-2蛋白的表达及CD105和CD34标记的MVD,并分析它们与胶质瘤临床病理因素的关系。结果:Ang-2在胶质瘤中的阳性表达率为62.2%,显著高于正常脑组织(P<0.05)。胶质瘤组织中,CD105、CD34标记的MVD均明显高于正常脑组织。Ang-2表达的阳性标记指数及CD105标记的MVD与胶质瘤病理分级密切相关(P<0.01),CD34标记的MVD与胶质瘤病理分级无明显相关(P>0.05)。Ang-2与CD105、Ang-2与CD34均呈协同表达,CD105在胶质瘤中表达的特异性较CD34高。结论:Ang-2、CD105和CD34表达在促进胶质瘤的恶性演进中发挥重要作用;CD105较CD34更能准确反映肿瘤血管内皮细胞的增殖状态。

Objective ：To investigate the expessions of Ang -2, CD105 and CD34 and their biological significance in gliomas. Methods ：The expressions of Ang- 2 and MVD labeled by CD105, CD34 were examined by SP immunohistochemical staining in 45 human glioma specimens and 10 normal human brain tissue specimens. Results： The positive expression rates of Ang -2 in gliomas（62.2% ）was significantly higher than that of normal human brain tissues（ P 〈 0.05 ）. The microvessel density（MVD） marked by CD105 or CD34 in gliomas was obviously higher than in normal brain tissue（ P 〈 0.01 ）. The positive label index of Ang - 2 expression and CD105 - MVD were closely correlated with pathological grade of gliomas（ P 〈 0.01 ）, but no significance between CD34 - MVD and pathological grade （P 〉 0.05 ）. There was coexpression of Ang- 2 and CD105 or Ang -2 and CD34 in gliomas, the expression of CD105 had higher specificity in gliomas. Conclusion： Ang -2,CD105 and CD34 play important role in malignant progression of gliomas ;CD105 is more specific than CD34 to reflect the proliferation of endothelial cells.