饮酒法建立胎儿酒精中毒综合征大鼠模型

Ethanol administrastions in pregnant rats cause morphological changes in fetus

目的使用饮酒法建立胎儿酒精中毒综合征大鼠模型,评估动物模型的形态学变化。方法性成熟雌性大鼠在孕前饲以酒精溶液,受孕后分组,饮酒组在孕期G9-G12饲以20%(v/v)酒精溶液,对照组在孕期一直使用普通饮水饲养。评估各组胚胎和新生鼠的形态学变化。结果饮酒组新生大鼠的头部长度,头身全长,体重,双睑间距和睑裂长度均较对照组明显下降,G20期胚胎畸形的例数也明显增加。结论使用饮酒法建立的胎儿酒精中毒综合征大鼠模型表现符合酒精胚胎发育毒性损害的形态学改变。

Objective To establish a rat model of fetal alcohol syndrome and to understand the morphological changes of the rat model. Methods Twenty adult female rats were given drinking water with successively increased concentration of ethanol before pregnancy, starting at a concentration of 3 % and ending at 20 % （v/v）. Pregnant rats were randomly divided into two groups. Ethanol drinking group were provided liquid diet containing 20 % （v/v） ethanal and were maintained on this diet for 4 days. Control group received a normal diet without ethanol. Morphological characteristics of fetus and newborns in each group were evaluated. Results Head length, body length, body weight, interocular breadth and palpebral fissure length of newborn rats in ethanol drinking group were significantly decreased than in control group. There were three outcomes shown in fetus of ethanol drinking group ： normal fetus, malformed placenta with resorbed fetus and malformed fetus. Conclusion Morphological changes related to the developmental toxicity were found in rat model of fetal alcohol syndrome.