厨余垃圾高温堆肥中嗜热细菌种群结构分析

Analysis of thermophilic bacterial communities structure in high temperature composting of kitchen waste.

基于16SrDNA的PCR-变性梯度凝胶电泳(DGGE)技术和系统发育分析,对厨余垃圾高温堆肥中嗜热细菌种群结构进行研究.堆肥高温阶段(>50℃)持续7d,在此期间从堆体中采样并提取微生物总DNA,使用细菌通用引物GC-341F/907R从总DNA中成功PCR扩增出目标16SrDNA片段,对扩增16SrDNA进行DGGE分析,DGGE条带相似性Cs值分析和切胶测序,使用序列数据进行同源性分析并建立系统发育树.DGGE和相似性Cs值分析结果表明,堆肥高温阶段有着比较丰富的细菌多样性,同时存在明显的优势种群结构变化;切胶测序和系统发育分析结果表明,大部分高温阶段优势种群序列与芽孢杆菌属(Bacillus)和梭菌属(Clostridium)中的嗜热菌群具有较近的亲缘关系.

Using base on the polymerase chain reaction （PCR） of 16S rDNA-denaturing gradient gel electrophoresis （DGGE） technique and phylogenetic analysis, the thermophilic bacterial communities structure in high temperature composting of kitchen waste was studied. The composting high-temperature stage （〉50℃） continued 7 days, in this period, sampling stack body and collecting microbial total DNA of the object, 16S rDNA fragments were amplified using universal bacteria primers （GC-341F/907R）. The DGGE analysis was carried out on amplification of 16S rDNA. The DGGE twig belt similarity Cs value analysis and cut glue survey order, using order arrange data to carry out same source property analysis, and system phylogenetic tree was established. The analysis results of DGGE and similarity Cs value showed that the composting high temperature stage had more rich bacteria diversity and existed obvious superior community structure change.The analysis results of cut survey order and phylogenetic showed that large part of high temperature stage superior community order arrange possessed nearer blood relation with Bacillus and Clostridium.