生物强化技术改善太湖梅梁湾水源地水质的研究

Studies on the bioaugmentation techniques for improvement of source water quality in Meiliang Bay of Taihu Lake.

采用海绵状立方体新型载体ACP和PM2种材料,通过生物强化技术来改善太湖梅梁湾水源地水质.静态实验结果表明,生物强化技术对湖泊水体中的TN、NH4+-N、NO2--N、NO3--N、TP、PO43--P、Chl-a的平均去除率分别达到了83.13%、80.80%、70.57%、78.05%、62.71%、78.06%、84.77%.载体上生物量和生物活性的监测结果表明,载体ACP和载体PM具有很强的富集生物和提高生物活性的能力,富集后的生物量和生物活性能分别达到31.23,32.58μg/g、158.32,165.12μgTF/g.FISH检测技术发现,载体表面上富集硝化细菌(硝化菌、亚硝化菌)数量能分别达到6.4×1010个/g.PCR技术、16SrRNA分析表明,假单胞菌(Pseudomonas sp.)和芽孢杆菌(Bacillussp.)作为优势溶藻细菌富集,富集的丰度达到1.62%.

Two kinds of new type materials： carrier angular cubic polystyle （ACP） and carrier polystyle miture （PM） was adopted to improve source water quality in Meiliang Bay of Taihu Lake. The results of static treat showed that the average removal rate ofbioaugmentation technique on TN, NH4^＋-N, NO2^--N, NO^3--N, TP, PO4^3-P, Chl-a reached 83.13%, 80.80%, 70.57%, 78.05%, 62.71%, 78.06%, 84.77% respectively. The monitoring results ofbiomass and bioactivity on the carrier showed that carrier ACP and PM possessed very strong capacity on enrichment of biomass and bioactivity, after which it could reach 31.23,32.58μg/g and 158.32,165.12μgTF/g respectively. It was found through FISH inspect measuring techniques that the quantity of nitrification bacteria （Nitrobacter and Nitrosomonas） could reach 6.4×10^10 cell/g. Through PCR technique and 16S rRNA analysis, Pseudomonas sp. and Bacillus sp. as prior algae-dissoluble bacteria were enriched with the enriched degree 1.62%.