不同来源水体有机综合污染指标的三维荧光光谱法与传统方法测量的对比研究

Comparative Research on Determination of Water Integrated Organic Pollution Index with Three Dimensional Excitation-Emission Fluorescence Spectroscopy and Traditional Wet Chemical Methods

比较研究了科学岛、巢湖和太湖不同来源水样的三维荧光光谱、COD值和DOC值。结果表明巢湖过滤水样COD值与DOC值线性相关(r=0.928 9);三维荧光光谱法测得的科学岛水样溶解有机物(DOM)浓度、类蛋白荧光强度(Ipro-like)、类腐殖质荧光强度(Ihum-like)均与COD值线性相关(r分别为0.821 72,0.84651和0.836 89);而三维荧光光谱法测得的巢湖和太湖水样的DOM浓度、Ipro-like和Ihum-like则与COD值无明显线性关系。上述结论与前人研究结果产生分歧,文章对原因进行了分析,并阐述了利用荧光光谱法测量水体有机污染综合指标的可行性和必要前提。

Three dimensional excitation-emission fluorescence spectra, chemical oxygen demanding （COD） and dissolved organic carbon （DOC） for water samples from Science Island, Chaohu Lake and Taihu Lake were determined. The COD values of the samples from Chaohu Lake were linearly correlated with the DOC values. Relationships of dissolved organic matter （DOM） concentrations determined by three dimensional excitation-emission fluorescence matrix （3DEEM）, observed protein-like fluorescence intensity （Ipro-like） and humic-like fluorescence intensity （Ihumlike） to the chemical oxygen Demanding （COD） for all the samples from different sources were analyzed. Results indicated that DOM concentrations measured by 3DEEM and COD for the water samples from Science Island were well linearly related （r=0. 821 72）, and the linear relationships also existed between Ipro-like and COD （r=0. 846 51）, as well as Ihum-like and COD （r=0. 836 89）. However, for the water samples from Chaohu Lake and Taihu Lake, no linear relationships existed. The cause of the above difference between the water samples from Science Island and those from Chaohu Lake and Taihu Lake, and the feasibility and the limits of the integrated organic pollution measurement using three dimensional excitation-emission fluorescence spectroscopy were further discussed.