高效液相色谱法测定人血浆中莫达非尼及莫达非尼酸的浓度

RP-HPLC determination of modafinil and modafinil acid in human plasma

目的:建立高效液相色谱法同时测定人血浆中莫达非尼及莫达非尼酸的浓度,以适合于大样本的血浆样品测定需要。方法:采用600 μL甲醇沉淀200μL血浆样品蛋白,高速离心后取上清液20 μL进样分析。分析柱:Diamonsil C_(18)柱(200 mm×4.6 mm,5μm),流动相:0.02 mol·L^(-1)磷酸二氢钾缓冲液(用磷酸调 pH=2.6)-甲醇-乙腈(60:20:20),紫外检测波长:220nm,流速:1.0 mL·min(-1),柱温:室温,以卡马西平作内标,按内标法定量。结果:血浆中莫达非尼和莫达非尼酸的线性范围均为0.1～10.0μg·mL^(-1),回归方程分别为:Y=0.252X+0.0094(r=0.9999)和 Y=0.2102X+0.0084(r=0.9991),最低定量限为0.1μg·mL^(-1)。莫达非尼、莫达非尼酸及内标卡马西平的保留时间分别为14.81,19.86,23.09 min,日内和日间 RSD 均小于11.0%,提取回收率和方法回收率分别在86.9%～95.8%,86.9%～96.8%和88.4%～94.7%,88.3%～99.8%之内。结论:本法具有快速、简便、灵敏、准确等优点,适用于人血浆中莫达非尼,莫达非尼酸浓度测定及药动学研究。

Objective：To establish an HPLC method for the determination of modafinil and modafinil acid in human plasma. Methods： Plasma sample was deproteinzed by methanol, centrifuged and 20 μL of the supematant was directly injected into HPLC. Separation was achieved on a 5 μm reverse phase column （ Diamonsil C18 200 mm ×4.6 mm,5μm）, with a mixture of 0. 02 mol . L^ -1 potassium dihydrogen phosphate - methanol - acetonitrile （60： 20： 20） as mobile phase. The flow rate was 1.0 mL . min^-1. The UV detector was set at 220 nm. The internal standard method was used to quantify modafinil and modafinil acid. Results： The linear concentration ranges of the calibration curves for modafinil and modafinil acid were both 0, 1 to 10. 0 μg . mL^-1. The lowest concentration of detection in plasma was 0. 1 μg . mL^- 1. The retention time for modafinil, modafinil acid and internal standard （carbamazepine） were 14.81,19.86,23.09 min ,respectively. The intra - day RSD and inter - day RSD were less than 11.0%. The extraction recoveries and method recoveries were 86. 9% - 95.8%, 86.9% - 96. 8% and 88.4% - 94.7%, 88.3% -99. 8%, respectively. Conclusion：This method is simple, rapid, sensitive and accurate, and suitable for clinical practice application.