高效液相色谱法同时测定脑血栓片中丹参素、原儿茶醛、芍药苷、阿魏酸和丹酚酸B的含量

HPLC determination of danshensu,protocatechuic aldehyde,paeoniflorin,ferulic acid and salvianolic acid B in Naoxueshuan tablets

目的:建立同时测定脑血栓片中丹参素、原儿茶醛、芍药苷、阿魏酸和丹酚酸 B 含量的方法。方法:采用反相高效液相色谱法,色谱柱:Zorbax Extend-C_(18)柱(4.6 mm×150 mm,5 μm),流动相:1%甲酸甲醇溶液(A)-1%甲酸溶液(B),梯度洗脱[0～8 min,A-B(8:92);8～23 min,A-B(8:92)→A-B(40:60)],流速1.0 mL·min^(-1),柱温30℃,检测器:DAD 检测器,检测波长为280 nm。结果:丹参素、原儿茶醛、芍药苷、阿魏酸和丹酚酸 B 的线性范围分别为0.072～0.72μg(r=0.9999),0.04～0.4μg(r=0.9999),0.032～0.32μg(r=0.9999),0.002～0.02μg(r=0.9993),0.08～0.8μg(r=0.9997);平均加样回收率(n=3)分别为97.2%,97.7%,98.3%,98.3%,96.7%。结论:本方法简便快速,结果准确可靠,适用于脑血栓片的质量控制。

Objective：To develop a method for the determination the contents of danshensu, protocatechuic aldehyde,paeoniflorin, ferulic acid and salvianolic acid B in Naoxueshuan tablets. Method：The analytical column was a Zorbax Extend -C18（4. 6 mm × 150 mm,5 μm）. The mobile phase consisted of 1% formic acid methanol is solution （A） -1% aqueous formic acid （B） with the gradient elution [0→8 min,A- B （8： 92）;8→23 min,A- B （40：60） ]. The flow rate was 1.0 mL . min^-1 and the column temperature was 30 ℃. The detector was DAD. The detection wavelength was 280 nm. Results： The calibration curves of danshensu, protocatechuic aldehyde, paeoniflorin,ferulic acid and salvianolic acid B were in good linearity over the ranges of 0. 072 -0.72 μg （r = 0. 9999）, 0.04 -0.4 μg （r =0. 9999） ,0. 032 -0.32 μg （r =0. 9999） ,0. 002 -0.02 μg （r =0. 9993） ,0.08 -0. 8 μg （r = 0. 9997）, and the average recoveries （n = 3 ） were 97.2% ,97.7% ,98.3% ,98.3% ,96. 7% respectively. Conclusion：The method is simple, rapid, reliable and can be used for the drug control.