浙贝母低温解除休眠过程中过氧化物酶的电泳分析

Peroxidase Assay over the Course of Dormancy Relieving with Low Temperature in Fritillaria thunbergii Miq.by Elec trophoresis

为了探讨浙贝母低温解除休眠的生理机制，利用电泳方法研究了芽、鳞片近轴面表皮与鳞片内部组织３个部位在休眠解除过程中过氧化物酶同工酶的变化。发现芽和鳞片不同部位的同工酶谱带在解除休眠的不同时期均有变化，且芽和鳞片近轴面表皮的过氧化物酶活性明显高于鳞片内部组织。这些结果说明芽和鳞片都参与了休眠的解除。

In order to study the physiological basis of dormancy relieving with low temperature in Fritillaria thunbergii,isoperoxidase changes of bud,adaxial epiderm and inner tissues in scale were assayed by means of electrophoresis over the course of dormancy relieving.All the patterns in bud and different parts of scale change at different stages,and the peroxidase activity in bud and adaxial epiderm is higher than that in the inner tissue of scale.These results indicate both bud and scale join in the dormancy relieving.