液相色谱-质谱法快速筛选人尿中26种利尿剂

Screening for 26 Diuretics in Human Urine by Rapid HPLC-MS

采用Zorbax30×2.1mm,1.8μm短柱,对26种利尿剂的液相色谱-质谱的测定方法进行了研究。建立了6min快速分离检测人尿中26种利尿剂的测定方法,检测限低于5ng。该方法适用于兴奋剂的筛选及确证工作。

Diuretics are banned by International Olympic Committee and World Anti-Doping Agency due to their capacity of masking doping agents in urine, diuretics are also prone to be misused regarding weight categories, screening procedures to check their presence in urine in doping control are mandatory. A fast and sensitive liquid chromatography mass spectrometric （LC-MS） method for the screening of 26 diuretics in human urine was described. Analyses were performed on Agilent 1 100 quadrupolar instrument equipped with ESI-interface. 26 diuretics were analyzed in 6 min after liquid-liquid extraction with ethyl acetate in human urine by rapid resolution HPLC-MS with Zorbax 30 × 2.1 mm, 1.8 m column. The mobile phase consisted of formic ammonium-formic acid buffer （pH 3.5） and acetonitrile. In the full scan mode, the LOD of these substances were below 5 ng. The method was ap- plied to detect diuretics after the oral administration of several drugs and also detect routine samples. It is suitable for doping screening and confirmation in doping control.